ID A0A1E5UDA2_9FLAO Unreviewed; 258 AA.
AC A0A1E5UDA2;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=3-deoxy-manno-octulosonate cytidylyltransferase {ECO:0000256|HAMAP-Rule:MF_00057};
DE EC=2.7.7.38 {ECO:0000256|HAMAP-Rule:MF_00057};
DE AltName: Full=CMP-2-keto-3-deoxyoctulosonic acid synthase {ECO:0000256|HAMAP-Rule:MF_00057};
DE Short=CKS {ECO:0000256|HAMAP-Rule:MF_00057};
DE Short=CMP-KDO synthase {ECO:0000256|HAMAP-Rule:MF_00057};
GN Name=kdsB {ECO:0000256|HAMAP-Rule:MF_00057,
GN ECO:0000313|EMBL:OEL10886.1};
GN ORFNames=BHF72_0081 {ECO:0000313|EMBL:OEL10886.1};
OS Cloacibacterium normanense.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales; Weeksellaceae.
OX NCBI_TaxID=237258 {ECO:0000313|EMBL:OEL10886.1, ECO:0000313|Proteomes:UP000095601};
RN [1] {ECO:0000313|EMBL:OEL10886.1, ECO:0000313|Proteomes:UP000095601}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRS-1 {ECO:0000313|EMBL:OEL10886.1,
RC ECO:0000313|Proteomes:UP000095601};
RA Capua I., De Benedictis P., Joannis T., Lombin L.H., Cattoli G.;
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Activates KDO (a required 8-carbon sugar) for incorporation
CC into bacterial lipopolysaccharide in Gram-negative bacteria.
CC {ECO:0000256|HAMAP-Rule:MF_00057}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=3-deoxy-alpha-D-manno-oct-2-ulosonate + CTP = CMP-3-deoxy-
CC beta-D-manno-octulosonate + diphosphate; Xref=Rhea:RHEA:23448,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:37563, ChEBI:CHEBI:85986,
CC ChEBI:CHEBI:85987; EC=2.7.7.38; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_00057};
CC -!- PATHWAY: Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno-
CC octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-
CC deoxy-D-manno-octulosonate and CTP: step 1/1. {ECO:0000256|HAMAP-
CC Rule:MF_00057}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00057}.
CC -!- SIMILARITY: Belongs to the KdsB family. {ECO:0000256|HAMAP-
CC Rule:MF_00057}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OEL10886.1}.
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DR EMBL; MKGI01000071; OEL10886.1; -; Genomic_DNA.
DR RefSeq; WP_069799255.1; NZ_MKGI01000071.1.
DR AlphaFoldDB; A0A1E5UDA2; -.
DR STRING; 237258.SAMN04489756_10783; -.
DR KEGG; cnr:EB819_05485; -.
DR PATRIC; fig|237258.4.peg.280; -.
DR OrthoDB; 9815559at2; -.
DR UniPathway; UPA00358; UER00476.
DR Proteomes; UP000095601; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008690; F:3-deoxy-manno-octulosonate cytidylyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0033468; P:CMP-keto-3-deoxy-D-manno-octulosonic acid biosynthetic process; IEA:UniProtKB-UniRule.
DR GO; GO:0009103; P:lipopolysaccharide biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd02517; CMP-KDO-Synthetase; 1.
DR HAMAP; MF_00057; KdsB; 1.
DR InterPro; IPR003329; Cytidylyl_trans.
DR InterPro; IPR004528; KdsB.
DR InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR NCBIfam; TIGR00466; kdsB; 1.
DR PANTHER; PTHR42866; 3-DEOXY-MANNO-OCTULOSONATE CYTIDYLYLTRANSFERASE; 1.
DR PANTHER; PTHR42866:SF2; 3-DEOXY-MANNO-OCTULOSONATE CYTIDYLYLTRANSFERASE, MITOCHONDRIAL; 1.
DR Pfam; PF02348; CTP_transf_3; 1.
DR SUPFAM; SSF53448; Nucleotide-diphospho-sugar transferases; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00057};
KW Lipopolysaccharide biosynthesis {ECO:0000256|ARBA:ARBA00022985,
KW ECO:0000256|HAMAP-Rule:MF_00057};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_00057}; Reference proteome {ECO:0000313|Proteomes:UP000095601};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00057}.
SQ SEQUENCE 258 AA; 29189 MW; 917A023EA8230A08 CRC64;
MNNLGTRNSE LETLKIIAVI PARYAASRFP GKLMQILGDK TVIATTYQNV LETLLFDKVF
VATDSEIIFE EITKIGGKAV MTGEHETGSD RIAEAVQNID CDIVINVQGD EPFLKTEPLK
QLIQVFKNDV DKEISLASLK IRLKEKEEIE NPNNVKVITD LQNFAMYFSR SVIPYPRETS
VEHLYYKHIG VYAFRKEALL KFANLAMTPL EISEKIEAIR YLENGMKIKM IETDFIGVGI
DVPEDLEKAK AILSRDKS
//
