ID A0A1E7FR82_9STRA Unreviewed; 793 AA.
AC A0A1E7FR82;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=sn-1-specific diacylglycerol lipase {ECO:0000256|ARBA:ARBA00026104};
DE EC=3.1.1.116 {ECO:0000256|ARBA:ARBA00026104};
GN ORFNames=FRACYDRAFT_234313 {ECO:0000313|EMBL:OEU20682.1};
OS Fragilariopsis cylindrus CCMP1102.
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta;
OC Bacillariophyceae; Bacillariophycidae; Bacillariales; Bacillariaceae;
OC Fragilariopsis.
OX NCBI_TaxID=635003 {ECO:0000313|EMBL:OEU20682.1, ECO:0000313|Proteomes:UP000095751};
RN [1] {ECO:0000313|EMBL:OEU20682.1, ECO:0000313|Proteomes:UP000095751}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCMP1102 {ECO:0000313|EMBL:OEU20682.1,
RC ECO:0000313|Proteomes:UP000095751};
RG DOE Joint Genome Institute;
RA Mock T., Otillar R.P., Strauss J., Dupont C., Frickenhaus S., Maumus F.,
RA Mcmullan M., Sanges R., Schmutz J., Toseland A., Valas R., Veluchamy A.,
RA Ward B.J., Allen A., Barry K., Falciatore A., Ferrante M., Fortunato A.E.,
RA Gloeckner G., Gruber A., Hipkin R., Janech M., Kroth P., Leese F.,
RA Lindquist E., Lyon B.R., Martin J., Mayer C., Parker M., Quesneville H.,
RA Raymond J., Uhlig C., Valentin K.U., Worden A.Z., Armbrust E.V., Bowler C.,
RA Green B., Moulton V., Van Oosterhout C., Grigoriev I.;
RT "Extensive genetic diversity and differential bi-allelic expression allows
RT diatom success in the polar Southern Ocean.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty
CC acid + H(+); Xref=Rhea:RHEA:33275, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17389, ChEBI:CHEBI:17815,
CC ChEBI:CHEBI:28868; EC=3.1.1.116;
CC Evidence={ECO:0000256|ARBA:ARBA00024531};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33276;
CC Evidence={ECO:0000256|ARBA:ARBA00024531};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
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DR EMBL; KV784354; OEU20682.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1E7FR82; -.
DR EnsemblProtists; OEU20682; OEU20682; FRACYDRAFT_234313.
DR KEGG; fcy:FRACYDRAFT_234313; -.
DR InParanoid; A0A1E7FR82; -.
DR OrthoDB; 373802at2759; -.
DR Proteomes; UP000095751; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR CDD; cd00519; Lipase_3; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002921; Fungal_lipase-like.
DR PANTHER; PTHR45792; DIACYLGLYCEROL LIPASE HOMOLOG-RELATED; 1.
DR PANTHER; PTHR45792:SF8; DIACYLGLYCEROL LIPASE-ALPHA; 1.
DR Pfam; PF01764; Lipase_3; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 4: Predicted;
KW Hydrolase {ECO:0000313|EMBL:OEU20682.1};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000095751};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 21..46
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 73..94
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 169..197
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 485..622
FT /note="Fungal lipase-like"
FT /evidence="ECO:0000259|Pfam:PF01764"
SQ SEQUENCE 793 AA; 89940 MW; 720EBC40019A2C9F CRC64;
MPALQLFGQR TFFAGDDICT FLVLHALFRA FQIAVALSLI GITVFYNHNQ EIILLENGMN
CTVEGSTEDR MTYIYSSVSI VVAGMSIYLV VPMFHYSRKG TPTEPTLREP LRRLCYFDTT
VMNVLRIIVF VLGSRDIKGV MAYCRCISND ISVDTFVEVH ESCPRHAGFM TAVITLFCTH
IFDACMTILT FLYFTFCHEN LFINSQIEFV SSEQRCSICL RCCIGWASIF TCCLFGGDKA
VRGDYANFSM LLANYVNGKG ILDVTASDIS VGLHMLRKVR KRNRLETTNR LVREHSEISK
QKVVSSRHGS SDRLSLNDDK NYENKIVVKM STDGNSVDLV HDNCNSEDTK EEIYDNRARP
QNLPEHRQKL EILSSVYTRD VNLINEAAHF MIYAQAAYTV VSYMMENPVT GFVSLQLQML
RNWCVCHFPS RKESIVGDNF WQIQTIAFKA LSGLAVKDIV YANFCDTITE IPYIILLDHE
WKSVVVTIRG TITLESVLTD MNVAPEALTR LGEECGFDGD GLYCHTGMLA STEWLYRDMK
RHGKLNKILA EYEGYKLRIV GHSLGAGIAA ILSVLLRPKY PDLRCLAFSP PGCVMSENLA
DDISTFTTAF VVNDDIVART SIEGFEELRN SILEMICRIK IPKHMVTKVS KNYDDTTVDG
LSEAIDHILY DKDDINDSKF KQEIDEFQKH QIELKEKNKE SYIKLCPPGN IIQLFLTADV
RQSECITTHN GEEIGQYTAR WANRSDFEKI NISSHLISDH NTNGVKYKLH ELAIGKFGLH
PPFKPISDPD REI
//
