ID A0A1E7GC01_9EURY Unreviewed; 374 AA.
AC A0A1E7GC01;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 17.
DE SubName: Full=B12-binding domain/radical SAM domain-containing protein {ECO:0000313|EMBL:OEU42493.1};
GN ORFNames=BGV40_09190 {ECO:0000313|EMBL:OEU42493.1};
OS Methanosarcina sp. Ant1.
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX NCBI_TaxID=1882735 {ECO:0000313|EMBL:OEU42493.1, ECO:0000313|Proteomes:UP000175834};
RN [1] {ECO:0000313|EMBL:OEU42493.1, ECO:0000313|Proteomes:UP000175834}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Ant1 {ECO:0000313|EMBL:OEU42493.1};
RA Buongiorno J., Bird J.T., Krivushin K., Oshurkova V., Shcherbakova V.,
RA Rivkina E.M., Lloyd K.G., Vishnivetskaya T.;
RT "Draft Genome Sequence of Antarctic Methanogen Enriched from Dry Valley
RT Permafrost.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OEU42493.1}.
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DR EMBL; MDTP02000081; OEU42493.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1E7GC01; -.
DR Proteomes; UP000175834; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR023980; CHP04013_B12-bd/rSAM.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR NCBIfam; TIGR04013; B12_SAM_MJ_1487; 1.
DR PANTHER; PTHR43409:SF7; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE; 1.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Cobalt {ECO:0000256|ARBA:ARBA00023285}.
FT DOMAIN 1..105
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 138..374
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 374 AA; 41773 MW; B9862FE427851F35 CRC64;
MDVHFRYSKK NSYSFAVLSP LLPEAGFADR PIEGIMIYSF TTRQAAKVFM EVKNASTDSI
FIAGGPHPSG VPDETLEYFD YVVLGEGEET LPELVKVLKE GGDPRKVWGI AYRDSGTGKI
VTTPKRPYVN LDTYPCFDPK KLRSPIEISR GCPWGCKYCQ TPRLFGREIR HRSVDSILKN
AGHYNDLRFI ASNAFAYGSD GIHPRFDKVE KLLSALHALP DKKIFFGTFP SEVRPEFVTE
ESAELVRKYC ANDSLSLGAQ SGSDRILKEI RRGHTVEDTI SAVECCLEHE IVPAVDFIFG
LPTETEEDQE KSLDLVRWIC SKGGTVRAHY LTPLPGTPYS SAVPAEVNDG VRRELGKLAL
GGKLTGYWEK HRKT
//
