ID A0A1E7QK65_WOLPI Unreviewed; 383 AA.
AC A0A1E7QK65;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=Proline--tRNA ligase {ECO:0000256|ARBA:ARBA00019110};
DE EC=6.1.1.15 {ECO:0000256|ARBA:ARBA00012831};
DE AltName: Full=Prolyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00029731};
DE Flags: Fragment;
GN ORFNames=BIY23_04745 {ECO:0000313|EMBL:OEY86858.1};
OS Wolbachia pipientis.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rickettsiales;
OC Anaplasmataceae; Wolbachieae; Wolbachia.
OX NCBI_TaxID=955 {ECO:0000313|EMBL:OEY86858.1, ECO:0000313|Proteomes:UP000175679};
RN [1] {ECO:0000313|EMBL:OEY86858.1, ECO:0000313|Proteomes:UP000175679}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=wPpe {ECO:0000313|Proteomes:UP000175679};
RA Brown A.M., Wasala S.K., Howe D.K., Peetz A.B., Zasada I.A., Denver D.R.;
RT "Genomic evidence for plant-parasitic nematodes as the earliest Wolbachia
RT hosts.";
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-
CC tRNA(Pro); Xref=Rhea:RHEA:14305, Rhea:RHEA-COMP:9700, Rhea:RHEA-
CC COMP:9702, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:60039,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78532, ChEBI:CHEBI:456215;
CC EC=6.1.1.15; Evidence={ECO:0000256|ARBA:ARBA00000857};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OEY86858.1}.
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DR EMBL; MJMG01000003; OEY86858.1; -; Genomic_DNA.
DR RefSeq; WP_070064911.1; NZ_MJMG01000003.1.
DR AlphaFoldDB; A0A1E7QK65; -.
DR OrthoDB; 9809052at2; -.
DR Proteomes; UP000175679; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004827; F:proline-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006433; P:prolyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd00779; ProRS_core_prok; 1.
DR Gene3D; 3.40.50.800; Anticodon-binding domain; 1.
DR InterPro; IPR002314; aa-tRNA-synt_IIb.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR036621; Anticodon-bd_dom_sf.
DR InterPro; IPR002316; Pro-tRNA-ligase_IIa.
DR InterPro; IPR004500; Pro-tRNA-synth_IIa_bac-type.
DR InterPro; IPR033730; ProRS_core_prok.
DR NCBIfam; TIGR00409; proS_fam_II; 1.
DR PANTHER; PTHR42753; MITOCHONDRIAL RIBOSOME PROTEIN L39/PROLYL-TRNA LIGASE FAMILY MEMBER; 1.
DR PANTHER; PTHR42753:SF2; PROLINE--TRNA LIGASE, MITOCHONDRIAL-RELATED; 1.
DR Pfam; PF00587; tRNA-synt_2b; 1.
DR PRINTS; PR01046; TRNASYNTHPRO.
DR SUPFAM; SSF52954; Class II aaRS ABD-related; 1.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE 4: Predicted;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:OEY86858.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000175679}.
FT DOMAIN 33..327
FT /note="Aminoacyl-transfer RNA synthetases class-II family
FT profile"
FT /evidence="ECO:0000259|PROSITE:PS50862"
FT NON_TER 383
FT /evidence="ECO:0000313|EMBL:OEY86858.1"
SQ SEQUENCE 383 AA; 43878 MW; 1AE21D29B5907CC2 CRC64;
MYLSKYYLPT LKEAPSDAGI ISHKYSLRAG LIKQVVSGIY TWLPLGLKVL QNIEYIIREE
MEKSGAIEIL MPCIQPASLW QESGRYNDYG EEMLRINDRG QRNMLFGPTH EEVAIDLIRN
VVKSYKSLPL YLYQIQWKFR DELRPRFGVM RGREFLMKDA YSFDIDYENA INSYNLMYKT
YIKIFKRIGL TPIGVRADTG PIGGNLSHEF HILANTGEST LYYDNEFVKL LEDEEIESLR
HIYAVADDMY NIESCPISQE RLKISKGIEV GHIFYFGDKY SQPMKANITL KSGENVNIHM
GSYGVGVSRL VGAIIEVFHD SKGIIWPEAV APFKIGLINL HHRTEKCTKI ANELYKAFGG
NTVLYDDLEE STGIKFARMD LIG
//
