ID A0A1E7R313_9GAMM Unreviewed; 765 AA.
AC A0A1E7R313;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=phosphoenolpyruvate--protein phosphotransferase {ECO:0000256|ARBA:ARBA00012232};
DE EC=2.7.3.9 {ECO:0000256|ARBA:ARBA00012232};
GN Name=ptsP {ECO:0000313|EMBL:KAA8733835.1};
GN ORFNames=BJI46_04205 {ECO:0000313|EMBL:OEY93653.1}, F4V57_07230
GN {ECO:0000313|EMBL:KAA8733835.1};
OS Acinetobacter qingfengensis.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC Acinetobacter.
OX NCBI_TaxID=1262585 {ECO:0000313|EMBL:OEY93653.1, ECO:0000313|Proteomes:UP000185895};
RN [1] {ECO:0000313|EMBL:OEY93653.1, ECO:0000313|Proteomes:UP000185895}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ANC 4671 {ECO:0000313|EMBL:OEY93653.1,
RC ECO:0000313|Proteomes:UP000185895};
RA Capua I., De Benedictis P., Joannis T., Lombin L.H., Cattoli G.;
RL Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KAA8733835.1, ECO:0000313|Proteomes:UP000323633}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG 69710T {ECO:0000313|EMBL:KAA8733835.1,
RC ECO:0000313|Proteomes:UP000323633};
RA Pineiro-Iglesias B., Tunovic T., Unosson C., Inganas E., Ohlen M.,
RA Cardew S., Jensie-Markopoulos S., Salva-Serra F., Jaen-Luchoro D.,
RA Karlsson R., Svensson-Stadler L., Chun J., Moore E.;
RT "Draft genome sequence of various Type strains from the CCUG.";
RL Submitted (SEP-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-histidyl-[protein] + phosphoenolpyruvate = N(pros)-phospho-
CC L-histidyl-[protein] + pyruvate; Xref=Rhea:RHEA:23880, Rhea:RHEA-
CC COMP:9745, Rhea:RHEA-COMP:9746, ChEBI:CHEBI:15361, ChEBI:CHEBI:29979,
CC ChEBI:CHEBI:58702, ChEBI:CHEBI:64837; EC=2.7.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00000683};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the PEP-utilizing enzyme family.
CC {ECO:0000256|ARBA:ARBA00007837}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OEY93653.1}.
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DR EMBL; VXKN01000004; KAA8733835.1; -; Genomic_DNA.
DR EMBL; MKKK01000045; OEY93653.1; -; Genomic_DNA.
DR RefSeq; WP_070070468.1; NZ_VXKN01000004.1.
DR AlphaFoldDB; A0A1E7R313; -.
DR STRING; 1262585.BJI46_04205; -.
DR OrthoDB; 9765468at2; -.
DR Proteomes; UP000185895; Unassembled WGS sequence.
DR Proteomes; UP000323633; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW.
DR GO; GO:0016310; P:phosphorylation; IEA:InterPro.
DR Gene3D; 3.30.450.40; -; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR Gene3D; 3.50.30.10; Phosphohistidine domain; 1.
DR Gene3D; 1.10.274.10; PtsI, HPr-binding domain; 1.
DR InterPro; IPR003018; GAF.
DR InterPro; IPR029016; GAF-like_dom_sf.
DR InterPro; IPR008279; PEP-util_enz_mobile_dom.
DR InterPro; IPR000121; PEP_util_C.
DR InterPro; IPR023151; PEP_util_CS.
DR InterPro; IPR036637; Phosphohistidine_dom_sf.
DR InterPro; IPR006318; PTS_EI-like.
DR InterPro; IPR008731; PTS_EIN.
DR InterPro; IPR036618; PtsI_HPr-bd_sf.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR NCBIfam; TIGR01417; PTS_I_fam; 1.
DR PANTHER; PTHR46244:SF1; PHOSPHOENOLPYRUVATE-DEPENDENT PHOSPHOTRANSFERASE SYSTEM; 1.
DR PANTHER; PTHR46244; PHOSPHOENOLPYRUVATE-PROTEIN PHOSPHOTRANSFERASE; 1.
DR Pfam; PF01590; GAF; 1.
DR Pfam; PF05524; PEP-utilisers_N; 1.
DR Pfam; PF00391; PEP-utilizers; 1.
DR Pfam; PF02896; PEP-utilizers_C; 1.
DR PRINTS; PR01736; PHPHTRNFRASE.
DR SMART; SM00065; GAF; 1.
DR SUPFAM; SSF47831; Enzyme I of the PEP:sugar phosphotransferase system HPr-binding (sub)domain; 1.
DR SUPFAM; SSF55781; GAF domain-like; 1.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR SUPFAM; SSF52009; Phosphohistidine domain; 1.
DR PROSITE; PS00742; PEP_ENZYMES_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Phosphotransferase system {ECO:0000256|ARBA:ARBA00022683};
KW Pyruvate {ECO:0000313|EMBL:OEY93653.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000185895};
KW Sugar transport {ECO:0000256|ARBA:ARBA00022597};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:OEY93653.1};
KW Transport {ECO:0000256|ARBA:ARBA00022448}.
FT DOMAIN 22..169
FT /note="GAF"
FT /evidence="ECO:0000259|SMART:SM00065"
SQ SEQUENCE 765 AA; 85443 MW; B9C4B6DA598B5A51 CRC64;
MANTMQLETL RRIVQEVDAS PSLHEALDVM VDQVAQAMNV EVCSIYLLDE RNHRYILMAS
HGLRPESVGN VSLDISEGLV GLVGQREEIV NLENASKHER FIYLPETGEE IYNSFLGVPV
MYRRKVMGVL VIQNKESHDF SEAAESFLVT LCAQLSGAIA HAHAAGNIEI FRKSTSGPSY
QLYQGVAGAD GIALGRAVIL YPAADLSAIP DREAEDISEE LVLLNNAVAA VRGDIKNLDA
KMQDSLMSEE RALFSVFLRM LDDTALPAEI STMIRDGHWA QGAVRRVIEK HVNIFAQMED
DYLRERVSDL KDLGRRILAY LQTADTSHKE ITEDTILIGE EISTAALVEM PIHNISAIVT
TEGAANSHMV IVARALGIPT VVGVTELPIH TLDDAEIIVD AYQGRVFLHP PRRLRTRYKE
IKKEEEQIAK DLKQYETKSA ITPDGHTLPL FVNTGLMIDV VRGVQRGAKG VGLYRSEIPF
MLRERFPGEE EQRLIYRQQL SHFANKPVVM RTLDIGADKD LPYFSIDEEN SALGWRGIRF
TLDHPEIFSS QIRAMLKASI GLNNLHILLP MVTSVSELEE VLYLLDRDLA AVQEEEQVKI
NKPKIGIMIE VPSVLYQINE FAELVDFFSV GSNDLIQYLL AVDRNNPRVA SVYSHFHPAI
IRALTRLIQE CHKFDKPVSI CGEMAGNPLT AILLMAMGFN TLSMSSSNIL RVRKAICHVP
LSDAKRLLDE ALVIDNPLII KSMLEHYFRT HGLADMVKST RVVSV
//
