ID A0A1E8E547_9GAMM Unreviewed; 278 AA.
AC A0A1E8E547;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=Methylenetetrahydrofolate reductase {ECO:0000256|RuleBase:RU003862};
DE EC=1.5.1.54 {ECO:0000256|RuleBase:RU003862};
GN Name=metF {ECO:0000313|EMBL:QIV92762.1};
GN ORFNames=BJN41_01150 {ECO:0000313|EMBL:OFE44757.1}, GVU25_08115
GN {ECO:0000313|EMBL:QIV92762.1};
OS Acinetobacter towneri.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales; Moraxellaceae;
OC Acinetobacter.
OX NCBI_TaxID=202956 {ECO:0000313|EMBL:OFE44757.1, ECO:0000313|Proteomes:UP000186931};
RN [1] {ECO:0000313|EMBL:OFE44757.1, ECO:0000313|Proteomes:UP000186931}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=5-2Ac02 {ECO:0000313|EMBL:OFE44757.1,
RC ECO:0000313|Proteomes:UP000186931};
RA Barbosa B., Fernandez-Garcia L., Gato E., Leao R., Albano R., Fernandez B.,
RA Fernandez-Cuenca F., Marques E., Tomas M.;
RT "Genome of airborne Acinetobacter sp. 5-2Ac02 in the hospital environment:
RT Species near to Acinetobacter towneri.";
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:QIV92762.1, ECO:0000313|Proteomes:UP000502691}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=205 {ECO:0000313|EMBL:QIV92762.1,
RC ECO:0000313|Proteomes:UP000502691};
RA Zhou K., Cheng Y., Chen Y.;
RT "Identification of a novel tigecycline resistant gene in Acinetobacter
RT towneri.";
RL Submitted (JAN-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-
CC methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH;
CC Xref=Rhea:RHEA:19821, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636,
CC ChEBI:CHEBI:18608, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.5.1.54;
CC Evidence={ECO:0000256|ARBA:ARBA00034420};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:19823;
CC Evidence={ECO:0000256|ARBA:ARBA00034420};
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974,
CC ECO:0000256|RuleBase:RU003862};
CC -!- PATHWAY: Amino-acid biosynthesis; L-methionine biosynthesis via de novo
CC pathway. {ECO:0000256|ARBA:ARBA00034478}.
CC -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|RuleBase:RU003862}.
CC -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase family.
CC {ECO:0000256|ARBA:ARBA00006743, ECO:0000256|RuleBase:RU003862}.
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DR EMBL; MKQS01000001; OFE44757.1; -; Genomic_DNA.
DR EMBL; CP048014; QIV92762.1; -; Genomic_DNA.
DR RefSeq; WP_004972760.1; NZ_MKQS01000001.1.
DR AlphaFoldDB; A0A1E8E547; -.
DR STRING; 202956.BJN41_01150; -.
DR GeneID; 64223525; -.
DR eggNOG; COG0685; Bacteria.
DR UniPathway; UPA00193; -.
DR Proteomes; UP000186931; Unassembled WGS sequence.
DR Proteomes; UP000502691; Chromosome.
DR GO; GO:0005829; C:cytosol; IEA:InterPro.
DR GO; GO:0004489; F:methylenetetrahydrofolate reductase (NAD(P)H) activity; IEA:UniProtKB-EC.
DR GO; GO:0009086; P:methionine biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR CDD; cd00537; MTHFR; 1.
DR Gene3D; 3.20.20.220; -; 1.
DR InterPro; IPR029041; FAD-linked_oxidoreductase-like.
DR InterPro; IPR003171; Mehydrof_redctse-like.
DR InterPro; IPR004620; MTHF_reductase_bac.
DR NCBIfam; TIGR00676; fadh2; 1.
DR PANTHER; PTHR45754; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR PANTHER; PTHR45754:SF3; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR Pfam; PF02219; MTHFR; 1.
DR SUPFAM; SSF51730; FAD-linked oxidoreductase; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605};
KW FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|RuleBase:RU003862};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW ECO:0000256|RuleBase:RU003862};
KW Methionine biosynthesis {ECO:0000256|ARBA:ARBA00023167};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU003862}.
SQ SEQUENCE 278 AA; 30637 MW; 060C5C05890393EE CRC64;
MTKQVPISFE FFPSKTDVGA EKLKVVHQEL QLLNPEFFSI TYGAGGSTRE RTLSTISDFN
GKGTPVAPHL SCIGDDKARI AELLDIYKAQ GIDRIVALRG DLPSGQVGLG ELPYASDLVR
FIREHSGDHF HIEVAAYPEM HPQADHFDQD IKNFVTKVNA GANAAITQFF FNPDAYFYFV
ERIQKQGVNI PVAPGIMPIT NASNLIRFAD GTGAEIPRWI RKQLATYGDD TASIKAFGHE
VIIKLCERLI AGGAPSLHFY TMNNTEPTRQ LVTDLGLA
//
