ID   A0A1E8FE97_9ALTE        Unreviewed;       428 AA.
AC   A0A1E8FE97;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   27-MAR-2024, entry version 27.
DE   RecName: Full=TRAM domain-containing protein {ECO:0000259|PROSITE:PS50926};
GN   ORFNames=BFC17_19545 {ECO:0000313|EMBL:OFI34254.1};
OS   Alteromonas lipolytica.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Alteromonadales;
OC   Alteromonadaceae; Alteromonas/Salinimonas group; Alteromonas.
OX   NCBI_TaxID=1856405 {ECO:0000313|EMBL:OFI34254.1, ECO:0000313|Proteomes:UP000176037};
RN   [1] {ECO:0000313|EMBL:OFI34254.1, ECO:0000313|Proteomes:UP000176037}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JW12 {ECO:0000313|EMBL:OFI34254.1,
RC   ECO:0000313|Proteomes:UP000176037};
RA   Wu Y.-H., Cheng H., Xu X.-W.;
RT   "Alteromonas lipolytica, a new species isolated from sea water.";
RL   Submitted (SEP-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC       ProRule:PRU01024}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:OFI34254.1}.
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DR   EMBL; MJIC01000014; OFI34254.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1E8FE97; -.
DR   STRING; 1856405.BFC17_19545; -.
DR   Proteomes; UP000176037; Unassembled WGS sequence.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 2.40.50.1070; -; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR030390; MeTrfase_TrmA_AS.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   InterPro; IPR002792; TRAM_dom.
DR   InterPro; IPR010280; U5_MeTrfase_fam.
DR   NCBIfam; TIGR00479; rumA; 1.
DR   PANTHER; PTHR11061:SF49; 23S RRNA (URACIL(1939)-C(5))-METHYLTRANSFERASE RLMD; 1.
DR   PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR   Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR   PROSITE; PS50926; TRAM; 1.
DR   PROSITE; PS01230; TRMA_1; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01024}; Reference proteome {ECO:0000313|Proteomes:UP000176037};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01024};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01024}.
FT   DOMAIN          1..53
FT                   /note="TRAM"
FT                   /evidence="ECO:0000259|PROSITE:PS50926"
FT   ACT_SITE        382
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT   ACT_SITE        382
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         258
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         287
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         308
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT   BINDING         356
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ   SEQUENCE   428 AA;  47161 MW;  D92A090040A245A4 CRC64;
     MASITIDSWD PRGRGISRQH QPVLFVDGAL PGETCQVTVT ASKKQLCEGK VTKVLEAAPQ
     RQQPFCPVFS QCGGCQLQYV QREAAVSWRQ QALDSQIRHQ YGLDTLPWQP PVFSPEEQYR
     RKTRLAIDAR KGKPFSLGFR SARSDSVVNI SQCPVLEPEL NQLLPALHAV LPALKGRTAI
     GHVSLLKADN GLGVTLRFTQ PASSEDRALL TDFAREHKLN LRLDFGEHSE SLHAPVSELR
     CQTADGLSLA VTTEDFVQVN GAVNLAMIDQ AIQWLAPSDA DKVLDLFSGL GNFSLPLAKR
     VSEVTAVEGV STMVQRGEKN AHQQGINNIH WRTADLSNSQ ELNKLAVKKY DKILLDPSRE
     GAFAVCQQIA TSKVTSVVYV SCNPATFIRD LGPLLKDGWQ ISKIGLMEMF PYTQHIELMA
     LMERAGKG
//