ID A0A1F2P4P0_9EURY Unreviewed; 413 AA.
AC A0A1F2P4P0;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=tRNA pseudouridine synthase Pus10 {ECO:0000256|HAMAP-Rule:MF_01893};
DE EC=5.4.99.25 {ECO:0000256|HAMAP-Rule:MF_01893};
DE AltName: Full=tRNA pseudouridine 54/55 synthase {ECO:0000256|HAMAP-Rule:MF_01893};
DE Short=Psi54/55 synthase {ECO:0000256|HAMAP-Rule:MF_01893};
GN Name=pus10 {ECO:0000256|HAMAP-Rule:MF_01893};
GN ORFNames=ENI32_01110 {ECO:0000313|EMBL:HEC56476.1}, SBU_001060
GN {ECO:0000313|EMBL:OFV66123.1};
OS Candidatus Syntrophoarchaeum butanivorans.
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanosarcinales; Methanosarcinales incertae sedis; ANME-2 cluster;
OC Syntrophoarchaeum.
OX NCBI_TaxID=1839936 {ECO:0000313|EMBL:OFV66123.1, ECO:0000313|Proteomes:UP000185779};
RN [1] {ECO:0000313|EMBL:OFV66123.1, ECO:0000313|Proteomes:UP000185779}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BOX1 {ECO:0000313|EMBL:OFV66123.1};
RA Laso-Perez R., Richter M., Wegener G., Musat F.;
RT "Microbial consortia oxidize butane by reversing methanogenesis.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:HEC56476.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HyVt-386 {ECO:0000313|EMBL:HEC56476.1};
RX PubMed=31911466;
RA Zhou Z., Liu Y., Xu W., Pan J., Luo Z.H., Li M.;
RT "Genome- and Community-Level Interaction Insights into Carbon Utilization
RT and Element Cycling Functions of Hydrothermarchaeota in Hydrothermal
RT Sediment.";
RL mSystems 5:e00795-e00719(2020).
CC -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil-54 and
CC uracil-55 in the psi GC loop of transfer RNAs. {ECO:0000256|HAMAP-
CC Rule:MF_01893}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(54) in tRNA = pseudouridine(54) in tRNA;
CC Xref=Rhea:RHEA:57876, Rhea:RHEA-COMP:10193, Rhea:RHEA-COMP:14141,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_01893};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA;
CC Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01893};
CC -!- SIMILARITY: Belongs to the pseudouridine synthase Pus10 family.
CC {ECO:0000256|ARBA:ARBA00009652, ECO:0000256|HAMAP-Rule:MF_01893}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OFV66123.1}.
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DR EMBL; DRIE01000015; HEC56476.1; -; Genomic_DNA.
DR EMBL; LYOR01000004; OFV66123.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F2P4P0; -.
DR STRING; 1839936.SBU_001060; -.
DR PATRIC; fig|1839936.3.peg.1070; -.
DR Proteomes; UP000185779; Unassembled WGS sequence.
DR Proteomes; UP000885936; Unassembled WGS sequence.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.70.2510; -; 1.
DR Gene3D; 3.30.70.3190; -; 1.
DR HAMAP; MF_01893; Pus10_arch; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR005912; Pus10.
DR InterPro; IPR039894; Pus10-like.
DR InterPro; IPR048741; Pus10-like_C.
DR InterPro; IPR004114; THUMP_dom.
DR NCBIfam; TIGR01213; pseudo_Pus10arc; 1.
DR PANTHER; PTHR21568:SF0; TRNA PSEUDOURIDINE SYNTHASE PUS10; 1.
DR PANTHER; PTHR21568; UNCHARACTERIZED; 1.
DR Pfam; PF21238; Pus10_C; 1.
DR Pfam; PF02926; THUMP; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR PROSITE; PS51165; THUMP; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|HAMAP-Rule:MF_01893};
KW Reference proteome {ECO:0000313|Proteomes:UP000185779};
KW RNA-binding {ECO:0000256|HAMAP-Rule:MF_01893, ECO:0000256|PROSITE-
KW ProRule:PRU00529};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_01893}.
FT DOMAIN 35..166
FT /note="THUMP"
FT /evidence="ECO:0000259|PROSITE:PS51165"
FT ACT_SITE 235
FT /note="Nucleophile"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01893"
FT BINDING 298
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01893"
FT BINDING 369
FT /ligand="substrate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01893"
SQ SEQUENCE 413 AA; 47045 MW; FFFBFE466DD8BF54 CRC64;
MVEEDILSTA ERILTYGPIC DNCLGRQFAK LSGGLTNRER GHAIRTVLRM IHDDRTQPPQ
GCWVCLGLFE DSELSRWADM IEEALRGVEC RTFLIGTRMS GLLAENEETL WGKSGARHAE
PLKSELNREV GKIVSERMGL EVEFKHPDVL LILDLPRNKV DLRINPVFVF GRYLKLVRGI
PQTRWPCRRC HGKGCERCNF TGKLYKESVE ELIGEVAVRL FDGEGYTFHG AGREDIDARM
LGSGRPFILE IKSPKVRRID LKMLEEGVDR EKVQLLDLRY IDGSKVEVIK SASPWKRYRV
RVRLGSSDKK RLETALKEIS GRVIEQRTPT RVLHRRADIV RRRRVIDARL IRVEDGSAII
EILSDSGLYV KELISGDDGR TMPNLSMIMG EDLAVEELDV IEVGKIKGVE IQE
//
