ID A0A1F3EBA9_9BACT Unreviewed; 774 AA.
AC A0A1F3EBA9;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=A2X13_06690 {ECO:0000313|EMBL:OFX52470.1};
OS Bacteroidetes bacterium GWC2_33_15.
OC Bacteria; Bacteroidota.
OX NCBI_TaxID=1797324 {ECO:0000313|EMBL:OFX52470.1, ECO:0000313|Proteomes:UP000177978};
RN [1] {ECO:0000313|EMBL:OFX52470.1, ECO:0000313|Proteomes:UP000177978}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OFX52470.1}.
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DR EMBL; MENN01000003; OFX52470.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F3EBA9; -.
DR Proteomes; UP000177978; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 6..291
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 292..573
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 27..34
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 774 AA; 88483 MW; 8ACDACB48430D291 CRC64;
MYEFLNELNE AQREAVVNYS GASLVIAGAG SGKTRVLTYR IAYLLAKEIP ARQILALTFT
NKAAAEMKSR IAKMAGNDVA RFLWMGTFHS IFARILRNES GKLGYSSNYT IYDPIDTKSL
IKKIIKELNL DDKIYKSNEV YGRISSAKNN LITAKAYSSN NQIIIQDQKN NRSRITDIYT
IYSNRCKKAD AMDFDDLLLN TNILFRDFPE VLEKYQNMFK YILVDEYQDT NFAQYLIVNK
LSSKNKNICV VGDDAQSIYS FRGAKIENIL NFKNDYPGYK LFKLEQNYRS TQTIVNAANS
LIKKNKEQIP KKVFSANSVG EKIKVVEAET DKDEAYKIAR IIQDVIEEKN YDNKDFAILY
RTNAQSRSFE EAFRRFNIPY KVYGSISFYQ RKEIKDILAY FRLSINHNDE EALTRVINYP
ARGIGQTTLE RIEETAKNEN LTTWDILTQI QKNGLGLNSG TQSKLNSFKE LIEKFSEGIK
QNDAYDAAIL IAKESGIIDE FKNPESHEEQ TRLENLEELL NGIKEFVDEQ NDTDLLPTLD
KYMENVALLT NQDNEKDDDF NKVTIMTIHS AKGLEFKHVF LVGLEEDLFP SSMSSGTQKE
LEEERRLCYV ALTRAEETAV ISFAKERYKW GVPSSCRPSR FIKEIDEQFL ELPDSFLETP
FPLITKNDLP GFSSKPKFIK NDKPGINGNL QSVNKRLVKI SSNQNNHTNQ ESEIIPDDPG
LIAEGMVVRH SKFGLGEVLQ IEGVAPNSKA TVYFEVVGEK QLLLKFAKLQ IVKK
//
