ID A0A1F3H541_9BACT Unreviewed; 451 AA.
AC A0A1F3H541;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 24-JAN-2024, entry version 16.
DE RecName: Full=Citrate synthase {ECO:0000256|PIRNR:PIRNR001369};
GN ORFNames=A2W86_03710 {ECO:0000313|EMBL:OFX86206.1};
OS Bacteroidetes bacterium GWD2_45_23.
OC Bacteria; Bacteroidota.
OX NCBI_TaxID=1797330 {ECO:0000313|EMBL:OFX86206.1, ECO:0000313|Proteomes:UP000178053};
RN [1] {ECO:0000313|EMBL:OFX86206.1, ECO:0000313|Proteomes:UP000178053}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16;
CC Evidence={ECO:0000256|ARBA:ARBA00000308};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle.
CC {ECO:0000256|ARBA:ARBA00005163}.
CC -!- SIMILARITY: Belongs to the citrate synthase family.
CC {ECO:0000256|ARBA:ARBA00010566, ECO:0000256|PIRNR:PIRNR001369}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OFX86206.1}.
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DR EMBL; MENT01000008; OFX86206.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F3H541; -.
DR UniPathway; UPA00223; -.
DR Proteomes; UP000178053; Unassembled WGS sequence.
DR GO; GO:0036440; F:citrate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR024176; Citrate_synthase_bac-typ.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR PANTHER; PTHR11739; CITRATE SYNTHASE; 1.
DR PANTHER; PTHR11739:SF4; CITRATE SYNTHASE, PEROXISOMAL; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PIRSF; PIRSF001369; Citrate_synth; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; Citrate synthase; 1.
PE 3: Inferred from homology;
KW Transferase {ECO:0000256|PIRNR:PIRNR001369}.
FT ACT_SITE 326
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
FT ACT_SITE 387
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
SQ SEQUENCE 451 AA; 50784 MW; E9876FD9A4F7CA15 CRC64;
MDNEQLTKIL SDSIAFTSNI DKELFPKMGV KRGLRNEDHS GVLAGLTRVG DVVGYERQED
GTLKPIPGKL YYRGLDVEEL VKGIQSENRL GFEETAYLLL SGKLPTKEEL SAVNSLLAQT
MPLNHTATMN ILSLKGKNIM NILARSVLEL YAYDENPDDI SPENLVRQSV NLIAKFPTII
AYAYQVLRHD QQGRSLHIRH PYEDRSVAEN FLFMMKGRDK YTELDIKILD LALILHAEHG
GGNNSTFSVR VTSSTETDTY SAIAAGIGSL KGPLHGGANI KVQGMLEDMK KNVNDWKSVA
QIDDYLLKIL RKEAYDRTGL IYGIGHAVYT ISDPRADLLK SMARDLAVEK GRLEEFEFME
LIEQRAVEVF LGFKKETAKA RTCINVDFYS GFVYDAIGLP SEVFTPLFAM ARIVGWSAHR
IEELNFTSKR LIRPAYKNVR EKQPFVPINE R
//
