ID A0A1F3LD68_9BACT Unreviewed; 509 AA.
AC A0A1F3LD68;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=A2X18_08665 {ECO:0000313|EMBL:OFY38736.1};
OS Bacteroidetes bacterium GWF2_40_14.
OC Bacteria; Bacteroidota.
OX NCBI_TaxID=1797347 {ECO:0000313|EMBL:OFY38736.1, ECO:0000313|Proteomes:UP000176561};
RN [1] {ECO:0000313|EMBL:OFY38736.1, ECO:0000313|Proteomes:UP000176561}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OFY38736.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MEOK01000038; OFY38736.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F3LD68; -.
DR STRING; 1797347.A2X18_08665; -.
DR Proteomes; UP000176561; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 11..149
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 164..475
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT COILED 472..506
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 509 AA; 57336 MW; 68488D037057BD09 CRC64;
MATKTTQAEI NRIVWKACDT FRGVIDPSQY KDYILTMLFL KYVSDVHKSK FHEHLQKYDG
NLERAERAMR HERFIVPKTS SFDFLYEFRN ESNIGELINI ALADLEDANR EKMSSEDGSG
IFRNIDFNSS NLGDAKDKNT RLKNLILDFS DEKMIFDESH LEGNDIIGDA YMYLVSTFAS
ESGKKAGDFF TPAEVSTLLA KLTKSKPGAR ICDPTCGSGS LLIKAGMEVD GDNFSLYGQE
LNGSTWALAM MNMFLHGFDS AIIRWGDTLR TPKLKEGDLL MKFDTVVANP PFSLDKWGAD
EAEHDSYQRF WRGVPPKSKG DWAFISHMIE VAHEKTGKVG VVVPHGVLFR GSSEGKIRKQ
TVEENILEAV IGLPANLFFG TGIPAAIVIF NKGKKNKNVL FIDASKEFEN GKNQNKLRSQ
DIEHIVSTYC KFSAGELKEG TAEDKYAYVA TPAEIAENDY NLNIPRYVDT FEEEEEIDIV
AVQKEIDTLE IELKEVQAKM KQYLAELGF
//
