ID A0A1F3NXR2_9BACT Unreviewed; 1030 AA.
AC A0A1F3NXR2;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=A3G23_13275 {ECO:0000313|EMBL:OFY69045.1};
OS Bacteroidetes bacterium RIFCSPLOWO2_12_FULL_37_12.
OC Bacteria; Bacteroidota.
OX NCBI_TaxID=1797365 {ECO:0000313|EMBL:OFY69045.1, ECO:0000313|Proteomes:UP000176497};
RN [1] {ECO:0000313|EMBL:OFY69045.1, ECO:0000313|Proteomes:UP000176497}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OFY69045.1}.
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DR EMBL; MEPC01000039; OFY69045.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F3NXR2; -.
DR STRING; 1797365.A3G23_13275; -.
DR Proteomes; UP000176497; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 16..344
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 345..639
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 37..44
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1030 AA; 119223 MW; CC1ED5447AFF9945 CRC64;
MPPDSKYHQS FSAELKKLNP AQKKAVDSLE GPVMVIAGPG TGKTQILSAR IGKLLSHADL
QVQPHEILCL TYTDAATIAM RERLVSLIGP IAYNLHIYTF HAFCNQIIQE NQEYFGFKDL
QPVSDLEKFE LVEKMLMDLP VNHPLKRFKG DYHSDIINLL KLFSIMQKEG WTPEFISQKI
DEYLKGLPDK EEFRYKKKTK DANVGDLKVA RIREETEKKE RLRSAVFELK NYRTFMMQAS
RYDYDDMINW VNDGFAKKPD LLSSYQERYQ YFLVDEYQDT NGSQNEILKK LVSFWDNPNV
FIVGDDDQSI YRFQGANVEN MEDFATKYSG SITKITLVEN YRSTQKILNT ATSFIKRNNK
RLSGIMPGID KNLKASNIKI GSSVTMPAIL QYPNIYHEYA DLAHEIMRLL ENGANPSEIA
VIYRQHIQPD PLLKYLNHKK VPVLVKRKEN ILSAVFIKKI IRLLSYLSKE YEKPGSGNIL
LFELLHADFF GINAGDIALM TLECHKQKIH LRDMMASRER MFKTGISGIE NISRIHDDFT
FWTGAIANLT LQGWFEKIIT RGGILSFILK SPDKYYLMNL LTAFFDFIKS ETAKNPSMHV
REFLLILEKM EKHEIALEII RITGIGEGVN FLTAHGSKGL EFHHVFLIGC TANIWDSAGG
RMPFSFPDNL IIKNFSDEEE AEEARRLFYV ALTRAKEGLI ISFSNQNFNE KGLEKSRYVA
ELMESDDITF ENRNLSEDEL VEFQSLAMME EDAPEIELLD KIHLDELLKK YSLSPTHLSS
YIDCPVSFYY KHLIQVPTAK NEALGFGSAI HHALEKIFSV MQKTNYIFPV LTEFITYFHE
GMKRNKECFT EAEWKNRIDY GEKILPEYYE KYVRTWHKNV LPEKHYPNLF LDGIPINGII
DKIEIEGSAV NVVDYKTGNV ENGIKKLVRP DAETEPYGGD YWRQLVFYKI LLDTKKSLPG
IVISGEIDFI EKHKKKDDFI KEKVIFTSED VKFVTNLIKD VYQKIMNREF SKGCGKEECG
WCNFVRYWMR
//
