ID A0A1F4PIC0_9BACT Unreviewed; 730 AA.
AC A0A1F4PIC0;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=A3F66_00215 {ECO:0000313|EMBL:OGB83438.1};
OS candidate division TM6 bacterium RIFCSPHIGHO2_12_FULL_32_22.
OC Bacteria; Candidatus Dependentiae.
OX NCBI_TaxID=1802375 {ECO:0000313|EMBL:OGB83438.1, ECO:0000313|Proteomes:UP000178609};
RN [1] {ECO:0000313|EMBL:OGB83438.1, ECO:0000313|Proteomes:UP000178609}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGB83438.1}.
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DR EMBL; METJ01000072; OGB83438.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F4PIC0; -.
DR Proteomes; UP000178609; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Coiled coil {ECO:0000256|SAM:Coils};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 11..286
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 287..559
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT COILED 490..545
FT /evidence="ECO:0000256|SAM:Coils"
FT BINDING 32..39
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 730 AA; 85107 MW; 3B78B9BAE9367F37 CRC64;
MKQKFNEFLK TELNENQLKA VEEKNGSFLV IAGAGSGKTR VITARITNLI LNHNVHPSEI
LALTFTNKAA REMKERIEKF LSEETNLPFI GTFHSFCLLT LKKYKSFHKI ENFSILDDED
QLKLIKLILN KFSLNKQLNP RQVQYQISTL KNNLDSSLNK IEDKLLVEIL EAYEKEKRNS
RCLDFDDLLI EVVKLFKTNK IFKENFQATV RHILVDEYQD TNNVQHELLK EMALNKNFAL
DSICAVGDED QSIYSWRGAV VDNINHFKND FKNTKLIKVE QNYRSVNQIL DVANDVIKNN
KNRNEKKLWS NKKADNRIIR TTSFSGTKEA ELVAKYIKLA HKKDKQNSIA ILYRTHFQSR
LIEEALLRES INYKIIGGVQ FYERKEIKDI LAYLKIIVNP FDKISLFRIL NCPVRGLGSK
TEEIINDEWL LEPLLNYKEL FKKLDADKKL PAKKIEVLND FVKILDNFNF TDNTTTVIKE
LINKLEYLTY LKSEFEKKDA ESRIENLNEL IRASKYFEDQ NINSIKEFLD EVALIQDQLN
KKSDEEIQVQ LMTIHAAKGL EFDHVIIVGL EEGLLPGNKA LEKEELVEEE RRLFYVGITR
AKEYLIISNA KFRNHFGQTG NQIRSRFFDE ISDQKVHNFM HTNYNDHELT ALFSNFLNLA
PQATFKPSVM TFQSQSPKLV SEWKKNHFAK HKIFGIGLIK EIEKKPESTI ITLEFKTGPK
KIDSKFLEKI
//
