ID A0A1F4ZAT9_9BACT Unreviewed; 945 AA.
AC A0A1F4ZAT9;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=A2989_00820 {ECO:0000313|EMBL:OGD03358.1};
OS Candidatus Amesbacteria bacterium RIFCSPLOWO2_01_FULL_48_25.
OC Bacteria; Candidatus Amesbacteria.
OX NCBI_TaxID=1797259 {ECO:0000313|EMBL:OGD03358.1, ECO:0000313|Proteomes:UP000177080};
RN [1] {ECO:0000313|EMBL:OGD03358.1, ECO:0000313|Proteomes:UP000177080}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGD03358.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MEXN01000007; OGD03358.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F4ZAT9; -.
DR STRING; 1797259.A2989_00820; -.
DR Proteomes; UP000177080; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF48; ATP-DEPENDENT HELICASE_NUCLEASE SUBUNIT A; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 5..292
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 293..575
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 26..33
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 945 AA; 107351 MW; E9BEB924F5E9C2F7 CRC64;
MSEEKTLNKQ QLEAVTHSSG PLLIIAGAGT GKTTVVTERV KWLIAQGLAQ PSEILCLTFT
EKAAGQMQER IDVALPYGYT QMWVSTFHSF CDRILREEGL HLGLDTNYKL LTAPDAVSLL
KNHLFDFDLN YYRPLGNPAK FLTAALQHFN RLQDEDVSPS QYLAWSKKKD SNYQELAGLY
SDYSDLKVKE GVLDFSDLIS YSLKLFRDRP SVLKLYQQKF KYILVDEFQD TNYAQNQLIN
LLASRNRNLT VVADDDQAIY RWRGAAISNV IQFRKNYSDA KLITLVQNYR STQGILDKAH
TLIQNNNPDR LEVKEKIDKK LTSVRKIKGE KIELLHFDRV ESEADAVAKK IKEILTNDSK
LTAKDFAILV RANAHAEPFV RALSHHGIPH QFLGPGRLFH QPEIKDLIAY LKVLYNFEDN
VSFYRVLAME FFSIPARDLA VITNLARKQN LSLFAASEQS DLPSVKLLVD LIDRHLHLVP
KETAGQILYD FLEKSGLLKA ILDYRTPIDE IKAQNIMKFF NKLKSFETQN SDASVHTIVD
WLNLSSDMGE SPQAAEVDWG QNDAVNILTV HSAKGLEFPV VFLVNLVVDR FPSRSRSEPI
PIPDALIKEI LPEGDSHLQE ERRLFYVGLT RAKDLLFLTA ADFYGEGKRI KKLSPFIAET
LGPIQSSAHP AIQPSLLDWQ PARIATQSVA GGKPIPITYL SYSQIQTFLD CPLHYKAKYI
LKIPTPTSAA SSFGNTIHAT LKEFFTNKGQ TDVLDLLKKN WSSEGYTSKK HEQAYFDRGQ
KYLTEYLEKN FDPKHLPIKL EEPFIVPLPA GRQVFLKIGG KIDRVDLLPN GKIEIIDYKT
SVTLPTQKDA DKDLQLSFYA LVATLLREPP FNRKPEDVLL SLYFFDQQKK ISTTRTSTQL
EEAKQQIMSY ADQISRSDFH CSGSQLCRIC DYKILCDYAQ VDNAQ
//
