ID A0A1F5EHA9_9BACT Unreviewed; 546 AA.
AC A0A1F5EHA9;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=A2442_01370 {ECO:0000313|EMBL:OGD66755.1};
OS Candidatus Campbellbacteria bacterium RIFOXYC2_FULL_35_25.
OC Bacteria; Candidatus Campbellbacteria.
OX NCBI_TaxID=1797582 {ECO:0000313|EMBL:OGD66755.1, ECO:0000313|Proteomes:UP000179003};
RN [1] {ECO:0000313|EMBL:OGD66755.1, ECO:0000313|Proteomes:UP000179003}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGD66755.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; MFAE01000014; OGD66755.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F5EHA9; -.
DR STRING; 1797582.A2442_01370; -.
DR Proteomes; UP000179003; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 12..241
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT BINDING 33..40
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 546 AA; 63764 MW; FA920A058C4CFBF5 CRC64;
MKDKEYYSNF ENQRDEHTQN ILGSESKNKI ILAGPGTGKS YLFQQICKNN IENDLNNNLT
LSFINELVND LSKDLHGLSD VKTLHSFALK NISKNTNFFM NLGDVIQEDY KIIKNKEVSF
KDIFYNLEEE KEDELNFYSE RRKFYNFFGP NCSIYTLIKI FENNEEKIPK YSQILIDEFQ
DFNKLESRLI DLLAEKNSMV IAGDDDQSLY SFKNANPQEI RDKHKSEEYT SFELPFCSRC
PEVIINSFHN IVDKAKAEGF LKDRISKEYK YFPSVEKDLL SSQNPKLIIN KGVYQNQIAF
NIEKEIKSIS DSGKKDFSVL VICSLKSQIK SLEKALRKKG FNNIESPQEK KGGDLIDGLK
LLQKDKECNL GWRIVSKIIL ENNNKDIKDI IKKSHSNLNK KFYELLDKND VKYIKQVLAI
FKKVNENKSL KKEEYQKILD CFGYNQENIV LEKLKEDFQD NNNQKTNYKN IPIKITTILG
SKGLTNDYVF LVNFDDGYLF GKEKKITDEN ICKFLVALTR AKKKIYLYAN NNKLPTFVNW
IDKNNY
//
