ID A0A1F5EJ08_9BACT Unreviewed; 1058 AA.
AC A0A1F5EJ08;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 13-SEP-2023, entry version 17.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=A2442_02845 {ECO:0000313|EMBL:OGD67419.1};
OS Candidatus Campbellbacteria bacterium RIFOXYC2_FULL_35_25.
OC Bacteria; Candidatus Campbellbacteria.
OX NCBI_TaxID=1797582 {ECO:0000313|EMBL:OGD67419.1, ECO:0000313|Proteomes:UP000179003};
RN [1] {ECO:0000313|EMBL:OGD67419.1, ECO:0000313|Proteomes:UP000179003}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGD67419.1}.
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DR EMBL; MFAE01000005; OGD67419.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F5EJ08; -.
DR STRING; 1797582.A2442_02845; -.
DR Proteomes; UP000179003; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 6..73
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1058 AA; 119991 MW; 139421DF5D8E8284 CRC64;
MNPEFTHLHT HSHYSLLTAL PKITDLVKTA KADGMTSLAL TDNGNMYGAI EFYKTCKKEG
IKPILGVDFY VAARTRHDKQ SGIDNYRSRL VMLAKNEAGY KNFLKLVTKS QIEGFYYKPR
IDKELIETYK EGLVCISPHF SGEISQALKM NNFAKAEEIA IWYKEKFGEN FYLELTHHPE
INGQIELKEK IIAFGKKMNI PLVAAHDVYY LKPDDKRARD ILMSIQSSQG GSFGGEKDDF
SFISQKQAQT YFKNTPEALV NTEKIANLCN LELDLDSWIF PDIDIPAGET YDSQLKKLSY
AGIEKRGLER TKEIEDRIEY ELEIIKNKGY SSYFLIVADL LKYAKGAGIY TNTRGSAAGS
LVSYLNFITK VNPIELKLPF ERFMNPQRPG IPDIDIDIAD IRRDDVIDYA RKKYGKKAVA
QIGTFGTMAA RGSVRDVTRA LGLPYSLGDQ VAKLIPMGSQ GFPMTIERAL EMEEDLRKLH
KQNKDVQEII KYAKKIEGCA RHISTHAAGV LISPTRIDDY SPVQFDKEEK IITQYDMYTG
DRDGVVNLPK FDILGIKNLS ILSNAVNLAK ELEGVEVDLD NIDLEDKKTY DMLSRGETLG
TFQLNGQGMT QYLKELKPED IWDIMAMVAL YRPGPMAFIP DYIKRKNNPE LVTYLHPELK
AILEPTYGVI MYQDDVMLIA VNLAGYTWGE ADKFRKAMGK KIPKMMQEQK EKFYKGCLEH
GLKEKIVKEL WESIETFAAY GFNKAHSASY GRIAYETSYM KANFPIEYLT ALLMADSGDT
EKVSEIISEC KRMEIPVLPP DINESFTDFT IVDYEGGKAI RFGLQTIKNF GEGIANIIIE
ERAKNGKFKS LEDFLERIQD RNLNKKSLEA LTMCGAMDKL EERGQILGNI PDLLNYNKEN
ANKPKEQDSL FGLLSSDSNI PKLKLKEFPP AETKDMLSWE KELLGLYISG HPLDKYKELL
KKEGKNIKSI KEKTLDGVET VIAGIIEEVK LILTKKEAKM AFVKVSDFSD SMEVVVFPKV
FDEYRDLLEA NKCVILQGKI SSRNGEKGFL VNTVKELL
//
