ID A0A1F6AP24_9BACT Unreviewed; 617 AA.
AC A0A1F6AP24;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=A2960_04720 {ECO:0000313|EMBL:OGG26252.1};
OS Candidatus Gottesmanbacteria bacterium RIFCSPLOWO2_01_FULL_39_12b.
OC Bacteria; Candidatus Gottesmanbacteria.
OX NCBI_TaxID=1798388 {ECO:0000313|EMBL:OGG26252.1, ECO:0000313|Proteomes:UP000176609};
RN [1] {ECO:0000313|EMBL:OGG26252.1, ECO:0000313|Proteomes:UP000176609}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGG26252.1}.
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DR EMBL; MFJR01000012; OGG26252.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F6AP24; -.
DR Proteomes; UP000176609; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 7..291
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 292..529
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 28..35
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 617 AA; 71536 MW; 8A6244F1D8978D55 CRC64;
MESNLLKTLN PIQQQAVITT EGPILVLAGA GSGKTKVLTY KATYLILVKM IEPDNILMLT
FTNKAANEMK ERISKLLITH NSQPTTNLPF AGTFHWLCAK ILRKEGSFIG IPPNYIIYDQ
QDQKEALKQV ITELNLDKKK FNPSLAAALI SEAKNELITA QEYPQYAHGY FQQEIAKIFL
AYQKVLRENN ALDFDDLILM TVNLFQKVDE VARIYQNKFR YVLVDEYQDT NRAQFILTKL
LAKLWRNLCV VGDASQSIYG WRGADYRNIF NFNKDYSDVK VFHLEQNYRS TQNILDAAYA
VISKNNSHPI LKLWTNQTSG EPIVVYQASS EHDEAEYIIN QIIEIKYRFP EFQYSDFAVL
YRTNAQSRVL EETLILRGIP YILVGGVRFY ERKEIKDVLA YLRLLVNPKD KISYKRIEKI
GKKRLEKFVV FESRYKEKIE SNNLTTLALL DEVILETEYL ELYDKDNEED LSRLENIKEL
RSVASEFTDL NLFLENVALV EQEHLPDHPL IGKKIKAAVT LMTLHAAKGL EFKIVFLVGM
EEGLFPHSRS LLEPLELEEE RRLCYVGITR AMKRLYLTFA QRRLFFGLRS SNAVSRFITE
IPEDLTQIHQ TYKFTTT
//
