ID A0A1F6AX13_9BACT Unreviewed; 969 AA.
AC A0A1F6AX13;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=A2973_01615 {ECO:0000313|EMBL:OGG29168.1};
OS Candidatus Gottesmanbacteria bacterium RIFCSPLOWO2_01_FULL_49_10.
OC Bacteria; Candidatus Gottesmanbacteria.
OX NCBI_TaxID=1798396 {ECO:0000313|EMBL:OGG29168.1, ECO:0000313|Proteomes:UP000176409};
RN [1] {ECO:0000313|EMBL:OGG29168.1, ECO:0000313|Proteomes:UP000176409}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGG29168.1}.
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DR EMBL; MFJZ01000057; OGG29168.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F6AX13; -.
DR STRING; 1798396.A2973_01615; -.
DR Proteomes; UP000176409; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 9..319
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 320..601
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 30..37
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 969 AA; 111504 MW; 8DF75DBD50B3C543 CRC64;
MAKTPVEERQ LNVEQRQAVT YGAGPLLIIA GAGTGKTTVV TERIKHLITS GKAHPQEILA
LTFTEKASRE MEERVDIALP YGTTQMWIST FHAFCDRVLR NEAINIGLDP GFRLMTEAET
ILFFRKNLFK FELKYFRPLG NPNKFIAGMI QHFSRLKDED VSPGQYLAWV SSKSKIQNPK
QIQNPKSQII NENKEEAEKY KELAQSYRTY EELKVKEGVA DYSDLISNTL KLFRTRKNIL
KRYQDQFKYI LIDEFQDTNF AQNEMAILLA GVRENVTVVG DDDQAIYRWR GAAISNIIQF
RKRFPKAKII VLTKNYRSTK EILDRSYDLI NNNNPDRLEV VEKIDKKLES IRRINGEKIE
VIYANRVEEE AEEVAKEIQT LNFKLKNDWK DYAILVRANN HAEPFVRALA RLGIPYQFLG
PGQLFRQPEI KDLIAYLKVL YNFEDSVAMY RVLTMDWVGI EPRDVAGIIN DSRKFGNSVF
ETCEASADEN VKKLVAMIHR HLGLIKKETA GQILYYFLQD TGLLQQLTVY KTMKEEKTAN
NIAKFFNKLK THETEHEDAS VYAVVDWIDM AMNLGESPLA TDTDWTENNA VNILTIHSAK
GLEFPVVFLV NLVSARFPTI ERKEQIPIPD ALVKEILPSG DFHLEEERRL FYVGMTRARD
LLYFSAAKYY GEGKREKKLS PFIGEAIGEM LSAISHQPSD KIKQLSFLEW KKGEEKEEET
KPVRQPVTYL SYSQLSTFET CPLQYKYRYI LRIPVPPSAA LTFGDTIHRT VRAFYELVKT
GEKPTKIMLL DLLDKHWASR GYGDRGYEEK MKIHGKELLD GFFEKGYDPA LIPKDLEVSF
KIRITPSLTL GGRIDRVDIL SDGTLEIIDY KTGQSPKNRD VTKDAQLTVY ALAASEPGIY
NKKPENVIVS FYFFEDQIKI SATRSLGQLE KEKKEIALKA DEISRSDFKP TPGKHCDFCE
FRLICEAWR
//
