ID A0A1F6N9U1_9BACT Unreviewed; 582 AA.
AC A0A1F6N9U1;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
GN ORFNames=A3I29_03430 {ECO:0000313|EMBL:OGH80707.1};
OS Candidatus Magasanikbacteria bacterium RIFCSPLOWO2_02_FULL_44_11.
OC Bacteria; Candidatus Magasanikbacteria.
OX NCBI_TaxID=1798689 {ECO:0000313|EMBL:OGH80707.1, ECO:0000313|Proteomes:UP000178726};
RN [1] {ECO:0000313|EMBL:OGH80707.1, ECO:0000313|Proteomes:UP000178726}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrB family.
CC {ECO:0000256|ARBA:ARBA00010708}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGH80707.1}.
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DR EMBL; MFQK01000034; OGH80707.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F6N9U1; -.
DR STRING; 1798689.A3I29_03430; -.
DR Proteomes; UP000178726; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00081; Hint; 1.
DR Gene3D; 3.40.50.670; -; 1.
DR InterPro; IPR002288; DNA_gyrase_B_C.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR001241; Topo_IIA.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR000565; Topo_IIA_B.
DR InterPro; IPR013759; Topo_IIA_B_C.
DR InterPro; IPR006171; TOPRIM_domain.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR PANTHER; PTHR45866:SF1; DNA GYRASE SUBUNIT B, MITOCHONDRIAL; 1.
DR PANTHER; PTHR45866; DNA GYRASE/TOPOISOMERASE SUBUNIT B; 1.
DR Pfam; PF00986; DNA_gyraseB_C; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR01159; DNAGYRASEB.
DR PRINTS; PR00418; TPI2FAMILY.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00433; TOP2c; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 312..335
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
FT DOMAIN 322..436
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
SQ SEQUENCE 582 AA; 68250 MW; 54A0478CF2EA74C5 CRC64;
MPLYRQTSRL GKRITIAGYE LVRDPKENRW IFTHLLADEY NLAQGTYSIN DGTHRHHVDY
NKRNNNPKNI KRLTKEGHLQ IHRDHVDKTL RRPDVIAKML AAKKSPAYRS KMSAAMKKIA
PLLRARAKKQ WSDPAYKDYM KEKFLTFYKT NEVYRKKNNA LLNEEQFKYW ADKTHRQKQA
ERVTAFFEKH PERREALQAE ATEQWKNVDL RVWRAEKTKA QWDNSFRAKR KRAYDQVYLQ
HSLKLLSDVY KTNGTIENYD MVRRSIKANK NYLKLSTIIQ RFFKNDQKEL LAAVQNYNHR
IVSIKKLTKR IPVYDIEVPN THNFALANGV FVHNSAKQGR DREHQAILPL RGKVLNVERA
RLDKILTNNE LKSLIIAMGT NIGEQFDITK LRYHRIVIMT DADVDGAHIR TLLLTFFYRY
YVELLKNGHI YIAQPPLYSL KYGKEMFYAY NDEEKAKVLK DLENRKVAKA ETKKAAKNDK
EIVEGEGENE SEADKAVEAE EKQAIMIGGT KVNIQRYKGL GEMNPDQLWD TTMDPKNRMM
KLVTVDDAEA ANETFEILMG DEVEPRKKFI QTYAKSVQNL DI
//
