ID A0A1F6QPW6_9BACT Unreviewed; 926 AA.
AC A0A1F6QPW6;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=A3F80_09715 {ECO:0000313|EMBL:OGI10456.1};
OS Candidatus Melainabacteria bacterium RIFCSPLOWO2_12_FULL_35_11.
OC Bacteria; Candidatus Melainabacteria.
OX NCBI_TaxID=1801609 {ECO:0000313|EMBL:OGI10456.1, ECO:0000313|Proteomes:UP000198261};
RN [1] {ECO:0000313|EMBL:OGI10456.1, ECO:0000313|Proteomes:UP000198261}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGI10456.1}.
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DR EMBL; MFRO01000007; OGI10456.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F6QPW6; -.
DR STRING; 1801609.A3F80_09715; -.
DR Proteomes; UP000198261; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 2..247
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 321..515
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 341..516
FT /note="Exonuclease"
FT /evidence="ECO:0000259|SMART:SM00479"
FT DOMAIN 685..890
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 926 AA; 105515 MW; 96EBDC1FE474E20A CRC64;
MFFAMERTNM RTLGGIPTWA TYGFFKAIFD LLDRVKPKAI AAAYDCKEPT FRHKEYEDYK
ANRPEEMPEE LSVQWPYIRE GLESFEIPIY ELPGYEADDV IGTLAKKATK DGFNVLILTG
DRDAFQLIND KTKVLVPSKG ELKEYNRQAV FDYWGIWPEQ VIDFKSLSGD ASDNIPGIKG
IGEKTAEKLL TEFGSLDNIF KNAKKIPQKG LQEKILTGEK SAYLSKRLAA IDTDVPIDIN
LKDAHLNVPD MDKLVGFLQK FEFNSFIKRL PTILAMFNGE KAIVEKPAIV KRQGQMRLDL
DNLNGKTDDP LDDLQTPDLK INIIDTEKEL EKIISVLKDK KFICIDLETT SVNSNICDIV
GISLSYFKDE KSKLEKSDGI KAYYIPLGHE NGKQLNIEHV LQILKLILED NEKLKIAQNC
KFEYKILKRH GINLGKNIYD TMLASYVMNP DEKHGLKDQA ARILGMRMQK IDELIGEGKK
QLSMAQVEIK KAAPYAISDA TYTLQLSKYY KSTLEQPLEN VLETIENPLV PVLSEMELNG
IRIDTKVLKD LSDIITKKAQ ELEEKIFKIA GEPFNINSSQ QLGKILFQKL GIEHEGKITK
SGQLSTDART LETLARDDKT GIIENILEYR QLSKLISTYT DSLPEQIDKK TKNIHCDFNQ
TITSTGRLSS SNPNLQNIPI RSEIGRKIRK AFIPSFDNGL ILSADYSQIE LRILAHMSED
PVLIEAFKAG EDIHKRTAME IYGVKEITPE MRGHGKTLNF ALIYQQGAYA TARQLDISQK
EAQKFIDKYF QTFKKVKPFF EKLLNHARDH GYAETIYGRR RYFKNLKSHI KTLQREDERA
ACNAPLQGTA ADIMKLAMIK VAKDLKEKNY KSKLILQVHD ELVLDVHPHE KEKIKTLVQE
GMELGQPLHV PLVVNLSWGK DWYECG
//
