ID A0A1F6RY51_9BACT Unreviewed; 891 AA.
AC A0A1F6RY51;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=A3J76_05815 {ECO:0000313|EMBL:OGI25568.1};
OS Candidatus Moranbacteria bacterium RBG_13_45_13.
OC Bacteria; Candidatus Moranbacteria.
OX NCBI_TaxID=1801635 {ECO:0000313|EMBL:OGI25568.1, ECO:0000313|Proteomes:UP000178026};
RN [1] {ECO:0000313|EMBL:OGI25568.1, ECO:0000313|Proteomes:UP000178026}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGI25568.1}.
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DR EMBL; MFRT01000024; OGI25568.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F6RY51; -.
DR Proteomes; UP000178026; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06140; DNA_polA_I_Bacillus_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 7..267
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 297..471
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 650..856
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 891 AA; 101791 MW; 9FBC8DDFE9887102 CRC64;
MPSSHKKRLI LIDSNALIHR AYHALPPLTT KEGELVNAVY GYTSVLLNVL NKFKPEYIVA
TFDLKEPTFR HKQFKDYKAT RVKAPDDLYA QIPRVKEIVK ALSIPIAEKE GYEADDLIAT
FARKTEKLHP DVEVIVVTGD LDTLQLVDEK IKVFALRRGM SDSVLYDKKS IMERYGLNPE
QMVDYKGLRG DPSDNLPGVK GVGEKTAADL LKKYKTLENV YKNLPEIKEG IRKKLERDKV
QAYFSKRLAR LVDNVPSNFD LEKARAEDFS RVKIINLLRE LEFFSLLRRL PGYEEHLKDI
EEKNELKEKK EKLTWEKISP HQMPEIKNRL SLVSDGEIGG GIRGVAVCQN QKSAFYISRI
DEKVKNIFSD GKIGKIGYDL KKEWKILDEN KISLRGIKFD VQLAAYLLNS GVNLSWEDLV
LRELGILVEK ENKKAGQTAL IFQEDEGAIE KICRRASYNL ALEEKLEEKL EEISREQIMA
EKEKTLRNLF ENLEMPLIEA LARMEKEGIR INKTVLHGIS EKLDRRVEKL EKAIQKMAGE
KFNVNSSVQL RDVLFEKLKI PTKNIKTGKT GYSTASPELQ KIKTLHPIVE KIEEYRELFK
LKTTYLDPLP TLTDKNSRLH TSFNQAVTAT GRLSSSEPNL QNIPIRTDLG QLIRTAFEAE
EGFRLVGVDY SQIELRIAAH LSGDVRMITT FINGEDIHTA TAARVNKLDL DKVTKKMRSA
AKALNFGIIY GMSTFGFAQS AGVDQDASRE FIRKYFENYP QVAQFLENVK ISAREKGFVE
TELGRRRYIP EINSTNIQLR NQAERMAVNM PIQGLAADII KLAMLAAEKM IRKKYPIAKM
VLQIHDELLF EVPEQEAEKF ARDIKIAMEN VYKLSVPLIA DVKIGDNWGE I
//
