ID A0A1F6UUU6_9BACT Unreviewed; 996 AA.
AC A0A1F6UUU6;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=A2814_02000 {ECO:0000313|EMBL:OGI61089.1};
OS Candidatus Nomurabacteria bacterium RIFCSPHIGHO2_01_FULL_38_19.
OC Bacteria; Candidatus Nomurabacteria.
OX NCBI_TaxID=1801732 {ECO:0000313|EMBL:OGI61089.1, ECO:0000313|Proteomes:UP000177869};
RN [1] {ECO:0000313|EMBL:OGI61089.1, ECO:0000313|Proteomes:UP000177869}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGI61089.1}.
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DR EMBL; MFTI01000006; OGI61089.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F6UUU6; -.
DR STRING; 1801732.A2814_02000; -.
DR Proteomes; UP000177869; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 12..339
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 340..622
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 33..40
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 996 AA; 116558 MW; 5EBD83C27F381FB0 CRC64;
MDLKKRFQEE YKTLNKAQRE AVNATDGPVM VVAGPGTGKT QILALRIANI LQKTDTKPEN
ILALTFTNTG VISMRERLFN IIGDTAYRTN IFTFHAFCEH IIKELPFYFE ELEGARVISD
LERVEIVESI IKENKFKDLV SFHDEFSFLN KIVGGILNIK KEGLSPAEFI EKLPAWKQTL
LAGEDLYYKK DFGKYKKGDL KPTEAEKVNK KISQAEELGE IFTFYQQELK KRGFYDFSDM
VLYVLAELVK NKNLKADIQE KYQYILVDEH QDTNEGQNTL MELLTDAPHL EGKPNIFTVG
DEKQSIYRFQ GASAETFSRF QSLYTEINSI KLTENYRSTQ NILDGAHSLI VKSKALEDSP
SLHSNIEEND KISICEFSNY KFEFLYLAEE VKRRIDAGVA PSEIAVLYRA NKNVEDIKTI
FDFYKIHYTI FSKDKILDDP NIRNLIYILK VIHNLNDDHN LGKSFFAKFL KFDSYDVIKI
LDKFKYSRNR NIKHIFAIMQ DSQTLNEIEV KNIKDFLNFA KTIKELKIES QNQNFPDFFK
IFLAKIGYLK YMLSSADSRL QIIKLDKLLD EIKRQNKTRR AYSLTDFIYF VDAFSKYNLD
ITSTAPEIIE GVSLMTAHGS KGQEFEYVYI INTTRKAWEG GGRRGNSVAL PIYQYDGDIE
DERRLFYVAM TRAKKFLNIS YARTDNDGRE HEESEFVKEI DPLFKEETKM KKFEEKNVEN
LNVFLNAGKK SASIFEPQYL KELFFKRGLN VSALNNYLSC PKKYLHKNLI RIPDVYSPTL
KFGDLIHKSL ELFFNASVRA EKILSKEILL EFFEKELIKT YFPEKEEEKF RKRGEKALSE
YYDEYSDIWT YKIKTEFNVK RDFELDNRET LKLTGFIDKV ETNDEDNTIN IVDYKTGRTF
SEKTKEEKAA YERQIVFYHL LLQDYDEGNL SVTKSILDFV EKNKKGKFEQ YSFEVTKEHL
DKLREEINLC AHEILSMEFL KKGCDKNDCE WCKIEK
//
