ID A0A1F6WXT7_9BACT Unreviewed; 988 AA.
AC A0A1F6WXT7;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=A3A91_03680 {ECO:0000313|EMBL:OGI86706.1};
OS Candidatus Nomurabacteria bacterium RIFCSPLOWO2_01_FULL_36_16.
OC Bacteria; Candidatus Nomurabacteria.
OX NCBI_TaxID=1801767 {ECO:0000313|EMBL:OGI86706.1, ECO:0000313|Proteomes:UP000177001};
RN [1] {ECO:0000313|EMBL:OGI86706.1, ECO:0000313|Proteomes:UP000177001}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGI86706.1}.
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DR EMBL; MFUR01000012; OGI86706.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F6WXT7; -.
DR Proteomes; UP000177001; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 10..331
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 332..600
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 31..38
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 988 AA; 113984 MW; 2AEC12FFA4B7CF19 CRC64;
MASIFKEEYN KLNQAQKEAV DSIDGPVMVV AGPGTGKTQI LALRIGRILS ETDTKSDGIL
CLTFTNSAVK AMRERLRKYI GSEANKVKVS TFHSFGMDML EKYFPILGLD KAPRIMDERG
DVTILCDKIL QNNDWEYLRP RSDTSRYFQD LKSLVSLLKR ERITPEDFEN TIKIEIENIK
KDPESFSSRG ESKGRLKKDV EKRIEGLERT LEAVKFYSLY EIEKKGKNLF DYDDILEALN
KIVEDSEEAK EYIKTNFLYV LIDEHQDSSN AQNKFLENVW GDVEKPNIFV VGDDRQLIYG
FGGASLEYFE NFKNAFGKAK LITLSENYRS TQNILDSSHN LLKSSISKVK LKSNHKENHP
IRLVEAYYER DEIISCALEI KKKLNSIDPN NMAILVPKNK QVRSAITILK DMGIPVSGGE
MVDFFESHEA ISFLRVLKII ENPSDGVALA ESFFDKNSMI SPLMAHEFLK NNKMRDFSLL
NAVGEKNNLF NSENKINLWL RKLKSWLDYS SLPLYSFIQK VGAEFLLDTA KNHEELIIRI
EVLRTVLHLV LMQNEKNSNL NLNNFLDFID RLQSYGENIP LAVFGADEGV KVRTIHGSKG
LEFDFVWIAH MDERSFSGRK VGGFSLPESI KERVETRDEE VLKRQLYVAI TRAKRFCAIS
YALKSYIGGD QELAGIVADI AHNFEKQNAD ETEKIILKSD ERIFVETKKG IEKNTKLDGL
IKLVARDYED KKVSVSLLNN FFECPWKWYF RNLLQLPELK SESLEFGNIV HSSIDAILKL
DKKPNKEELQ NIVLFQVKKS GFGDERKQKE LIKLALNIIS KWVDKRLSEI SKNRENEKSI
SVSDDRFPSL NIYGKLDLIE YLDKKNARVT DFKTGNVRKK SEIEKTDEEG RMSSYLRQLA
MYSYLIEQSS RNKLHVKESR LEFLETKDAR ETFYYKVITQ KEISLLVQDI KDYDGFLKSG
EWVGRPCNYN SYGKNTECEY CKMAEIYK
//
