ID A0A1F7XDN5_9BACT Unreviewed; 1165 AA.
AC A0A1F7XDN5;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=A2Z22_04655 {ECO:0000313|EMBL:OGM12435.1};
OS Candidatus Woesebacteria bacterium RBG_16_34_12.
OC Bacteria; Candidatus Woesebacteria.
OX NCBI_TaxID=1802480 {ECO:0000313|EMBL:OGM12435.1, ECO:0000313|Proteomes:UP000177053};
RN [1] {ECO:0000313|EMBL:OGM12435.1, ECO:0000313|Proteomes:UP000177053}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGM12435.1}.
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DR EMBL; MGFS01000001; OGM12435.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F7XDN5; -.
DR Proteomes; UP000177053; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR016195; Pol/histidinol_Pase-like.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF160975; AF1531-like; 1.
DR SUPFAM; SSF89550; PHP domain-like; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 6..73
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
FT COILED 947..975
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1165 AA; 132881 MW; 8FD322C87A9930AC CRC64;
MSNNFCHLHV HTEYSLLDGL SRISEIILKV KELGMDSIAV TDHGAMYGVI EFYKKCKREN
IKPIIGIELY TTNLDYKQKP EKGRVKNHHL ILLAKDNQGY RNLMNLTSIA HLEGYYYRPR
VDKETLKKYS QGLICTSACP QGEVSSALIE NDYKKAKEVA EWFVNVFGKD YYLEVQRHEA
EKFIESVESE QLKEDLLNQK ENEKNIFNGI KKLSRELGIP IVATNDVHYI NKNDALAQDA
LVCIATGKKV TDIKRLRFID VPTFYLRSTE EMNDLFSDLP EATENSLKIS RKCEVEIILD
KWFFPEYKLD KGKSPDSEME DRIWIGLKEK IKPITKEIKQ RLKYEIDIIQ KKKYSTYFLI
VSDMARWAFE QGIVTNTRGS AAGSLASYAL GIININPLDY DLPFERFLTP WRPSPPDIDF
DIADDRREEI IDYITNKYGK DRVAQICTFG RMLARGSVRD IARVLGYAYS IGDRISKNIP
MGSQGFPMNI ERALEVSSEL KELYKNDADA KKILDLAKQV EGNARHVSVH AAGLVIAPDM
IINFTPIQLD PEGKKIITQY DMDALDPNVS PNEAVGLLKF DLLGLRNLSI LGSAIKIVKA
IEKKDIDLTK IPLDDKKTFI MLSKGDTMGV FQLSGSGMTR YLKELKPTSI QDLMAMVALF
RPGPMAQIPE FIERKNNPEK IKYFDPRMQE YLKKSYGLLV YQDDVFATAI KIAGYTWEEA
DKFRKAVGKK IPKEMEKQKI KFIEGAVKNG MKKTKAEELF KLIEPFSGYG FNKAHAASYG
MLAYHTAYMK ANFPIEFMCA LLTAESNDTD KISTAVHECK RMNIEVLPPD INESRVDFTI
VSYKKSLSNK AIRFGLSAIK NVGKAAIEEI LKARKKGNFI SFADFLSKVE NRKVNKKVLE
SLIKVGAMER FGKRAYLLSR LEEIRSKVGK SKTNSNQQEL FSKDMVKESV EIKFLQEKEE
IEEFSEEEIQ SLERQLLGFS LSAKPVGDLI EPFLTSITHK IYEISANESL DKMVKVAAVI
SDIRIIVTKR SGQEMAFVRA EDDTGSIDLI VFPKIFAQTR DIWVENKPIL ITARVDSREE
SPTLIVETID TDESIKSKSE NLFIRIPKNV KVEQLKDLRS LLQNNPGNQQ VSLVFEGKDD
YLVKLDFGIS WDQNLAKKIN NILYH
//
