ID A0A1F8E289_9BACT Unreviewed; 724 AA.
AC A0A1F8E289;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:OGM94943.1};
GN ORFNames=A2610_02100 {ECO:0000313|EMBL:OGM94943.1};
OS Candidatus Wolfebacteria bacterium RIFOXYD1_FULL_48_65.
OC Bacteria; Candidatus Wolfebacteria.
OX NCBI_TaxID=1802561 {ECO:0000313|EMBL:OGM94943.1, ECO:0000313|Proteomes:UP000179057};
RN [1] {ECO:0000313|EMBL:OGM94943.1, ECO:0000313|Proteomes:UP000179057}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGM94943.1}.
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DR EMBL; MGIV01000010; OGM94943.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F8E289; -.
DR Proteomes; UP000179057; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:UniProt.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 2..268
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 481..688
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 724 AA; 81160 MW; C912B2B3A146D65F CRC64;
MKKLLLIDAN ALIHRAFHAI PNLTTPDGRP SGALYGLANV LIKIFREEHP EYAVAFFDRP
EPTFRKKMYA EYKAHRPKAP DELVSQIIEA HNLFDIFGVK TYEVPGFEGD DLIGTAAEQF
KGEKDVQVVI LTGDLDALQL VEGNKVVVET LKQGISETIT YDTTAVEARY GITPSQLADY
KGLVGDTSDN IAGVRGVGPK TAAAIIQEFS SLENFFATVK ASDSTDKKML SYKKIAEHEE
IAMLSKQLAT IRRDAPIGIT SINEIKYEQF PIEAIVPYLQ DLGFTSIIKR LAPEARETRG
TRQEVRETQG QLSFLDAQPT LSKTLLDDVE RPLVPILEEM KQWGIMIDRV RLAEKEVILD
RDIDALTKEL YREAGQVFNP NSPKQLLELL KTKYQLKIKS TNIDKIEQFR ATVPLVDLIL
KYRELFKVKS TYLEPLKRMS DPSTSSGQMG DSRIHPTFVQ LKAATGRITC EQPNLQNIPP
SVRDIFVAPA GSKLVSLDYS QIELRILASL TEDPEMLRAF KSNSDIHQIT ASKIYNVPLA
DVTPTMRKVA KTLNFGVIYG MGARALAQQS GLSPKEAKAF IEEYFKDFAT IKHWQEGILA
KVREQGFVEN INGRIRPLPE IVSFNKMLQS EAERMAINFP IQSVGADIIK SAMVKVKEHL
SAEGLWGADV KMLLTVHDEL VFEIKDTERL PQTVNEIKDI LESVYTLKVP LTVNVEIGDN
WGEL
//
