ID A0A1F8JQQ7_9BACT Unreviewed; 964 AA.
AC A0A1F8JQQ7;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 24-JAN-2024, entry version 18.
DE RecName: Full=Protease 3 {ECO:0000256|ARBA:ARBA00017565};
DE EC=3.4.24.55 {ECO:0000256|ARBA:ARBA00012449};
DE AltName: Full=Pitrilysin {ECO:0000256|ARBA:ARBA00033450};
DE AltName: Full=Protease III {ECO:0000256|ARBA:ARBA00031184};
DE AltName: Full=Protease pi {ECO:0000256|ARBA:ARBA00029597};
GN ORFNames=A3E26_03285 {ECO:0000313|EMBL:OGN64381.1};
OS Chlamydiae bacterium RIFCSPHIGHO2_12_FULL_49_32.
OC Bacteria; Chlamydiota.
OX NCBI_TaxID=1797604 {ECO:0000313|EMBL:OGN64381.1, ECO:0000313|Proteomes:UP000176567};
RN [1] {ECO:0000313|EMBL:OGN64381.1, ECO:0000313|Proteomes:UP000176567}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- FUNCTION: Endopeptidase that degrades small peptides of less than 7
CC kDa, such as glucagon and insulin. {ECO:0000256|ARBA:ARBA00002184}.
CC -!- SIMILARITY: Belongs to the peptidase M16 family.
CC {ECO:0000256|ARBA:ARBA00007261, ECO:0000256|RuleBase:RU004447}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGN64381.1}.
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DR EMBL; MGLX01000013; OGN64381.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F8JQQ7; -.
DR Proteomes; UP000176567; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.30.830.10; Metalloenzyme, LuxS/M16 peptidase-like; 4.
DR InterPro; IPR011249; Metalloenz_LuxS/M16.
DR InterPro; IPR011765; Pept_M16_N.
DR InterPro; IPR001431; Pept_M16_Zn_BS.
DR InterPro; IPR007863; Peptidase_M16_C.
DR InterPro; IPR032632; Peptidase_M16_M.
DR PANTHER; PTHR43690:SF18; INSULIN-DEGRADING ENZYME-RELATED; 1.
DR PANTHER; PTHR43690; NARDILYSIN; 1.
DR Pfam; PF00675; Peptidase_M16; 1.
DR Pfam; PF05193; Peptidase_M16_C; 2.
DR Pfam; PF16187; Peptidase_M16_M; 1.
DR SUPFAM; SSF63411; LuxS/MPP-like metallohydrolase; 4.
DR PROSITE; PS00143; INSULINASE; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 55..184
FT /note="Peptidase M16 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00675"
FT DOMAIN 209..388
FT /note="Peptidase M16 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF05193"
FT DOMAIN 394..677
FT /note="Peptidase M16 middle/third"
FT /evidence="ECO:0000259|Pfam:PF16187"
FT DOMAIN 691..862
FT /note="Peptidase M16 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF05193"
SQ SEQUENCE 964 AA; 109645 MW; 0A983C7AAAED6BB5 CRC64;
MIKSLLFFLF FSSLFASEKG YELIEDRAAL PLLNFSLSEK RVAKLRLDNG LEAYLVSDPG
TEEAAASLSV EAGSWDDPQE YPGMAHFLEH MLFMGTHAFP DEREYWQYIQ DHGGSANAFT
APDRTVYLFS IKPYAFEGAV ERFSHFFIDP LFSPACIHRE LHAVDQEHAK NIENDLWREW
MIFKECGNPD HPNAKFSTGN AKTLSGIPQE ALKKWYQSHY SARVMHLILL SPQPLEQLIS
LTSRLFSKIP SFPLEKNRID LPLASSKQRG HFTYISPIKD LRRLTLTWEI PSSFVLDEES
HAPSLISYAL SHGGERGLLQ KLKEENLVED LAIEQNRLGK KEMLFSLILE LTHQGLSQID
TVIARCFQAI ARLKCSGISP NLFQEMQKIS TLNYQYQSRE NAFAFVNRHG FSIVDESLST
YPEKSTIPSS YNPAALSLFI EQLTPQNCLF TLHADPSLTG QPTTAKEKWM EAEYALGEIS
KTKLLSWQEI HPHPHIRLPD ANPYLPENLA LKTPLPNAPS HSAPHLLYAG KDGKIYYAQD
TKYLVPECGI FFNLKSSQID GSPQRQMLLD LFILALQDQN SQQNFFLKKA GYSLLLFSDQ
MALKVELSGY SDKAPLILKE LFKKMKSLSI SSPQFIRYKE ELATCYENQS RNLPLMQALD
FASGLLTNFH PTSAEKLVAL RALTFEESLP IFKELFDTVY AEGMIFGNLT ETEALQLSTT
LKALFDAKPY LLADQKKREV LLLPKEEGPL LIEQKTERQG SGVLLIIDCG PFALENHAAE
SLLASLLQEA FFDTLRTKQQ TAYIAKAFKK EIEHHIFQCF AVQSSTHTPT ELLFRFELFL
EEFLRNFEAN LAPERFRALK EALAASLEMA PENLSSMTTL LHTLAFEHEG DFSFRKKRLE
GLKELSYEEL HSFARKHLSR HNGGRLAVLM EGAPAKETLF HYRSLTKKEL NEKRTYTAGK
EKNL
//
