ID A0A1F8M3N7_9CHLR Unreviewed; 824 AA.
AC A0A1F8M3N7;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE RecName: Full=Beta-mannosidase B {ECO:0000256|ARBA:ARBA00041069};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Mannanase B {ECO:0000256|ARBA:ARBA00041614};
GN ORFNames=A2Z71_02015 {ECO:0000313|EMBL:OGN93226.1};
OS Chloroflexi bacterium RBG_13_50_21.
OC Bacteria; Chloroflexota.
OX NCBI_TaxID=1797623 {ECO:0000313|EMBL:OGN93226.1, ECO:0000313|Proteomes:UP000177581};
RN [1] {ECO:0000313|EMBL:OGN93226.1, ECO:0000313|Proteomes:UP000177581}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC mannosidase B subfamily. {ECO:0000256|ARBA:ARBA00038429}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGN93226.1}.
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DR EMBL; MGMQ01000160; OGN93226.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F8M3N7; -.
DR STRING; 1797623.A2Z71_02015; -.
DR Proteomes; UP000177581; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProt.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 189..296
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 655..740
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 743..823
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 824 AA; 94729 MW; 1800538A10FA678B CRC64;
MNIQPLSGIW QFRQVGTNEW SPAKVPGCVH TNLMALGSIP DPFVADNEKR VKWVAEADWI
YQHSFSCSSE LLAEEKIFLV CDGLDTLASL VLNGHELGHT DNMFRRNEWE IKPLLNSAGE
NVLTITFASP VKFIAEKNAI REMPGPYHSM GGGPYIRKAP YQFGWDWGPQ LPPIGIWKDI
RLEGYSRGRI AEVHLRQQHD SHQVAIEVRV AVQRWVVAPL KISLHITTPA GEILDKETTI
LSEEDVMMKV PISNPQLWWP NGYGDHPLYQ VEVLLKGSDS ASEDLLDLRS YQIGLRTIEL
RQQEDEWGRS FVFVVNQIPI LAKGSNWAPA ESFPTRISDE YLEGLIRSAA ETHQNMLRVW
GGGFYEEERF FDLCDRYGIL VWQEFIFSCS AYPLNDPEFL ENVHVEAIQN IHRLRHRASL
ALWCGNNEME WGWVDWNWDR SQLQDLKVAY DQFFHHTLPE WCQAADPDHS YWPSSPSSDT
PFVDPNGQRQ GDAHYWDVWH GGKPFTSYRQ QYPRFMSEFG FQALPPLATI RTYADKADWN
MTSYIMEQHQ KNASGNSMMV RQMLDMFRLP KDFESLVYLS LVLQAEGIRY GVEHWRRHPD
RVAGILYWQL NDCWPVASWS SLDYFGRWKA LHYAARRFYA PIMLSIEDKP PEQAVFVSND
WLDPWEGNLK WSLEKLDGEI LTSGEAPVRA APQAATQLCK LNFSDHITDD NLRSLVFIAE
LWQTERLVSR QSKCFAPIKH LSLTDPAISV NLHSKDGELI AEMISHSLAL LVEVSLQGSD
VVFSDNYFNL PTGRKVQVSC PMPAGWTLSH AQKELHIRSI YDSY
//
