ID A0A1F8NST2_9CHLR Unreviewed; 824 AA.
AC A0A1F8NST2;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Beta-mannosidase B {ECO:0000256|ARBA:ARBA00041069};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Mannanase B {ECO:0000256|ARBA:ARBA00041614};
GN ORFNames=A2Y53_02495 {ECO:0000313|EMBL:OGO13939.1};
OS Chloroflexi bacterium RBG_16_47_49.
OC Bacteria; Chloroflexota.
OX NCBI_TaxID=1797639 {ECO:0000313|EMBL:OGO13939.1, ECO:0000313|Proteomes:UP000179729};
RN [1] {ECO:0000313|EMBL:OGO13939.1, ECO:0000313|Proteomes:UP000179729}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC mannosidase B subfamily. {ECO:0000256|ARBA:ARBA00038429}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGO13939.1}.
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DR EMBL; MGNG01000029; OGO13939.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F8NST2; -.
DR Proteomes; UP000179729; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProt.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 3.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 192..296
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 655..740
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 743..823
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
FT REGION 469..489
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 469..487
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 824 AA; 94465 MW; 0F82234EFDA76B40 CRC64;
MQIQPLTGNW QFRQVGTIEW LPANVPGSVH TDLMAIGGIP DPFVADNEKR VQWVAESDWI
YRHRFNCSPE LLTQDKILLV CDGLDTLATI VLNGHELAHT DNMFRRYQWE VKSILNANGV
NDLTITFSSP VKYIAEKQAV RALPGVPQAI PGGPHLRKPP YQFGWDWGPQ LPPIGIWKDV
RLEGYSEARL AEIHLRQDHA SGQVKVVARI AVQRWGRALL TAAIRITTPD GEIIEKEASI
TALDEVIVKV PIPKPELWWP NGYGGQPLYR VDVSLMRSDA SNAEPLDRHM YQVGLRTIEL
RQQEDQWGRS FVFVVNGVPI FIKGSNWAPA DSFPTKISDE YLEGLIRSAA ETHQNMLRVW
GGGFYEEERF YDLCDRYGIL VWQEFIFSCS IYPLDIPAFL ENVQVEAIEN LRRLRHRASL
ALWCGNNEME WGWVDWNWNR PELQDLKAAY DRFFHHTLPA WCQTEDPDHS YWPSSPSSGT
PFDDPNGQRQ GDSHYWDVWH GRKPFTSYRD QYPRFMSEFG FQALPPLATI RTYADEADWN
MTSYIMEQHQ KNASGNSLMV GQMLDTFRLP KDFVSLVYLS MALQAEGIRY GVEHWRRHPE
RVAGTLYWQL NDCWPVASWS SLDYFGRWKA LHYAARRFYA PIMLSIEDKP PEQAVYVSND
TLEPWEGTAN WSLETFDGEI LTSGQAPVRA AAQAATQICK LDFCNLISDD NVRKLVFIVE
LWHGDQLISR QTAGFAPIKH LSLTDPAVRV NLYSEKGQLI AELTSRSLAL LVEVSLTNTE
VVFSDNYFNL PPGRTTRISC PLPPGWSLRQ AKKEFHIRSI YDSY
//
