ID A0A1F8PIH2_9CHLR Unreviewed; 381 AA.
AC A0A1F8PIH2;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE SubName: Full=Heme d1 biosynthesis radical SAM protein NirJ1 {ECO:0000313|EMBL:OGO22601.1};
GN ORFNames=A2Z28_04950 {ECO:0000313|EMBL:OGO22601.1};
OS Chloroflexi bacterium RBG_16_51_9.
OC Bacteria; Chloroflexota.
OX NCBI_TaxID=1797645 {ECO:0000313|EMBL:OGO22601.1, ECO:0000313|Proteomes:UP000179905};
RN [1] {ECO:0000313|EMBL:OGO22601.1, ECO:0000313|Proteomes:UP000179905}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGO22601.1}.
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DR EMBL; MGNM01000075; OGO22601.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F8PIH2; -.
DR Proteomes; UP000179905; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd21123; SPASM_MftC-like; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR017200; PqqE-like.
DR InterPro; IPR007197; rSAM.
DR PANTHER; PTHR11228:SF7; PQQA PEPTIDE CYCLASE; 1.
DR PANTHER; PTHR11228; RADICAL SAM DOMAIN PROTEIN; 1.
DR Pfam; PF13353; Fer4_12; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR PIRSF; PIRSF037420; PQQ_syn_pqqE; 1.
DR SFLD; SFLDG01386; main_SPASM_domain-containing; 1.
DR SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 24..234
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 381 AA; 42722 MW; C393EBA7DC41795D CRC64;
MISITRLLCD EAGPGDNLRY ERNAAPRPVT VWNYTRQCNL NCLHCYATAD TRKNPDEMDT
KAGEAFIRDL AEFRIPVLLF SGGEPMLRKD LLSLAAFAKE QGVRPAISTN GTLITEDTAR
DLKRVGFAEV GISLDGIGAK NDYFRGKEGA FDSALRGIRA CIAQGLRVSL RQTLTLHNYQ
DVPAIFDLVE KEGVNRVCFY HLAYSGRGSG LVKEDISHEQ TRDVVDTICE RTIDLHKRGL
HKEILTVSSH TDGVYLYLKL REKDPKRAEK VLELLRISGG NQSGIKIGSV DDVGNVHADQ
FWWHHSLGNV RQRKFGDIWM DQTEPLLKGL KNRAGLLKGR CGRCHYVDIC NGNLRVRAEA
VYDDVWAQDP ACYLTEEEIT G
//
