ID A0A1F9VGA7_9BACT Unreviewed; 894 AA.
AC A0A1F9VGA7;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 24.
DE RecName: Full=Aconitate hydratase {ECO:0000256|RuleBase:RU361275};
DE Short=Aconitase {ECO:0000256|RuleBase:RU361275};
DE EC=4.2.1.3 {ECO:0000256|RuleBase:RU361275};
GN ORFNames=A3G41_08910 {ECO:0000313|EMBL:OGS01940.1};
OS Elusimicrobia bacterium RIFCSPLOWO2_12_FULL_59_9.
OC Bacteria; Elusimicrobiota.
OX NCBI_TaxID=1797952 {ECO:0000313|EMBL:OGS01940.1, ECO:0000313|Proteomes:UP000176685};
RN [1] {ECO:0000313|EMBL:OGS01940.1, ECO:0000313|Proteomes:UP000176685}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- FUNCTION: Catalyzes the isomerization of citrate to isocitrate via cis-
CC aconitate. {ECO:0000256|RuleBase:RU361275}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501,
CC ECO:0000256|RuleBase:RU361275};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185, ECO:0000256|RuleBase:RU361275}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGS01940.1}.
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DR EMBL; MGUX01000131; OGS01940.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1F9VGA7; -.
DR STRING; 1797952.A3G41_08910; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000176685; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd01586; AcnA_IRP; 1.
DR CDD; cd01580; AcnA_IRP_Swivel; 1.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR044137; AcnA_IRP_Swivel.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01341; aconitase_1; 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR PANTHER; PTHR11670:SF54; CYTOPLASMIC ACONITATE HYDRATASE; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|RuleBase:RU361275};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|RuleBase:RU361275};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|RuleBase:RU361275};
KW Lyase {ECO:0000256|RuleBase:RU361275};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT DOMAIN 73..564
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 694..820
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 894 AA; 97677 MW; 4540768CFD6CF260 CRC64;
MDSFSTLKTL KAGGSSYRIF SIRALAQAGL GDIGRLPYSI RVLLENLLRH EDGLSVARDD
IQALARWRPD QKPDKEIQFM PSRVLLQDFT GVPAVADLAA MRDAIAALGG DPQKINPLQQ
SDLVIDHSVQ VDSYGLRDAF RLNAEKEFKR NQERYKFLCW GQKAFNNFRV VPPDTGIVHQ
VNLEYLASVV YSRRTPEGLT AYPDTLVGTD SHTTMISGLG VLGWGVGGIE AEAAMLGQPI
AMLAPPVVGF KLTGALAEGA TATDLVLTVT QMLREKGVVG KIVEFYGAGL SALALTDRAT
LANMAPEYGA TTGFFPVDDE TLNYLRQTGR DPKQIRLVKA YLKEQGLFRT DDAPVPLYSD
SLELDLSRVE PSLAGPRRPQ DRVPLRNSKP EFEKALGAFW PKQTAATPAA AKVPVQMNGQ
RFELGHGSVV IASITSCTNT SNPQVMLQAG LLAQKAARAG LKSKPWVKTS LAPGSKVVMD
YLQKSKLLPA LESLGFYLVG YGCTTCIGNS GPLPDAVEKA IQAANLVAAC VLSGNRNFEG
RIHPSVRASY LASPPLVVAY ALAGRMDIDL YREPLGHDAS GKPVFLKDIW PSQKEVRLAV
QKFIKKEMFH KQYGKIFQGD KRWKTLRVPG GSRFAWSKDS TYIKKPPFFA GMTREPEKPR
PIAAARVLAL LGDSVTTDHI SPAGNIPADG PAGRYLIKQG VEKKDFNSFG ARRGNHEVML
RGAFSNIRLR NLLAPGTEGG WTRRLPDGEV MTIYDAAMSY AKEKTDLLIL AGKEYGSGSS
RDWAAKGAAL LGVRAVIAES YERIHRSNLV GMGILPLQFK LGENCRSLGL TGEETFDIEN
VAEDFKPGRE MEIACLAPGG GKNRFKVLCR IDTPKELEYY RHGGILKYAL RQLL
//
