ID A0A1G0WJN3_9BACT Unreviewed; 1010 AA.
AC A0A1G0WJN3;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 18.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=A2475_13595 {ECO:0000313|EMBL:OGV21439.1};
OS Ignavibacteria bacterium RIFOXYC2_FULL_35_21.
OC Bacteria; Ignavibacteriota; Ignavibacteria.
OX NCBI_TaxID=1798452 {ECO:0000313|EMBL:OGV21439.1, ECO:0000313|Proteomes:UP000177327};
RN [1] {ECO:0000313|EMBL:OGV21439.1, ECO:0000313|Proteomes:UP000177327}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family.
CC {ECO:0000256|ARBA:ARBA00006594}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGV21439.1}.
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DR EMBL; MHAY01000064; OGV21439.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G0WJN3; -.
DR STRING; 1798452.A2475_13595; -.
DR Proteomes; UP000177327; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR011639; MethylTrfase_TaqI-like_dom.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR025931; TaqI_C.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR PANTHER; PTHR33841:SF5; TYPE II METHYLTRANSFERASE M.HINDII; 1.
DR Pfam; PF07669; Eco57I; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR Pfam; PF12950; TaqI_C; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 338..469
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
FT DOMAIN 539..654
FT /note="Type II methyltransferase M.TaqI-like"
FT /evidence="ECO:0000259|Pfam:PF07669"
FT DOMAIN 778..941
FT /note="TaqI-like C-terminal specificity"
FT /evidence="ECO:0000259|Pfam:PF12950"
SQ SEQUENCE 1010 AA; 117724 MW; F9A5E7D804E15447 CRC64;
MADNIADKLS NLVTDFRLAN FRGLNEWQTR QTYIEPFWEI LGWNVRKQSE VQGEKTESSK
RPDYRFRKDN KTLFFVEAKK PNENLKENQN HIYQAKIYCW NGKVPLAVLT DFEEFRPFRA
YGLPKKDDRE KGIIKQFDMK CEEYPDRAYD LLNTFGKEAV YGGSLNEIIQ KPTQVELRDT
VDKHFLITLT KWRTDLAAQI AKINKFANEF DLAEAVQRIL DRLVFLRVLQ DRDIETEDFM
CGISKSKTDE PYSAFLKICK AMAPKYNGLI FNPHPLSENL KIEGRLFKKI VQELDFEESP
YRFDEIPVEM LGTIYERFLG DEISFSPRGK LVIDKKPEVR KAGGVYYTPQ YIVEYIVQNT
VGKLLEICKT PEDVAKLKIL DPACGSGSFL LGAFNLLIKW HEDYFSANPT KIIEKTGKQY
FNLAYLDDNN ITKLTAKMKG RILQNNLYGV DLDKQATEVA QMSLYIRVLE GLTGESIMQL
GFHEALLPRL DGNIKCGNSL IGPDFINEGF FKLPEREKHK INPFDWEEQF PFIKEKGGFD
AVIGNPPYLR VQGLQEYHSN IIQYLKNYYK SASTGNFDIY VIFIEKGFSL LNDRGYFGFI
VPHKFFPSQF GENIRELITS KNALEEIVHF GSNKVFEQAD TYTCLLFLSK KKNKEFKIIE
FGSGADLESE LNLIAKSKNK ESNKFKIGFI NHPEKSKDKW SFKSGETGGL IDKLLNSNFP
KLNEITEKIF VGLQTSADDI YVLKVKDNKY LNYKNLKSFK LFSKSLNEEV EIEKGLIKPF
LMGRDVYRYD DPIFRNYVIF PYTIINNSAV IMAKENIKEK FPNGWHYLLE NRKVLEGREN
GKMNHDKFYA YIYPKNLAYF EQEKILIREL GDKPNMFFDN ENFYHTAKIY SLLLKDKSNL
NYLYMLGILN SKILNFFIKN TSEIWGTSHK FKTQFLNPFP IPNLTDRKVK IDMVSLVDRM
LKLHKDKQTA SDFDRKQIDQ YIAKTDKEID KLVYELYTLT EEEIKVVEGI
//
