ID A0A1G1K260_9BACT Unreviewed; 526 AA.
AC A0A1G1K260;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 24-JAN-2024, entry version 17.
DE RecName: Full=Malate synthase {ECO:0000256|ARBA:ARBA00012636, ECO:0000256|RuleBase:RU000555};
DE EC=2.3.3.9 {ECO:0000256|ARBA:ARBA00012636, ECO:0000256|RuleBase:RU000555};
GN ORFNames=A3C35_00340 {ECO:0000313|EMBL:OGW86972.1};
OS Omnitrophica bacterium RIFCSPHIGHO2_02_FULL_46_11.
OC Bacteria; Candidatus Omnitrophota.
OX NCBI_TaxID=1801820 {ECO:0000313|EMBL:OGW86972.1, ECO:0000313|Proteomes:UP000176909};
RN [1] {ECO:0000313|EMBL:OGW86972.1, ECO:0000313|Proteomes:UP000176909}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00001699,
CC ECO:0000256|RuleBase:RU000555};
CC -!- PATHWAY: Carbohydrate metabolism; glyoxylate cycle; (S)-malate from
CC isocitrate: step 2/2. {ECO:0000256|RuleBase:RU000555}.
CC -!- SIMILARITY: Belongs to the malate synthase family.
CC {ECO:0000256|ARBA:ARBA00006394, ECO:0000256|RuleBase:RU000555}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGW86972.1}.
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DR EMBL; MHFF01000020; OGW86972.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G1K260; -.
DR STRING; 1801820.A3C35_00340; -.
DR UniPathway; UPA00703; UER00720.
DR Proteomes; UP000176909; Unassembled WGS sequence.
DR GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-UniPathway.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR CDD; cd00727; malate_synt_A; 1.
DR Gene3D; 3.20.20.360; Malate synthase, domain 3; 1.
DR Gene3D; 1.20.1220.12; Malate synthase, domain III; 1.
DR InterPro; IPR044856; Malate_synth_C_sf.
DR InterPro; IPR011076; Malate_synth_sf.
DR InterPro; IPR006252; Malate_synthA.
DR InterPro; IPR019830; Malate_synthase_CS.
DR InterPro; IPR001465; Malate_synthase_TIM.
DR InterPro; IPR048355; MS_C.
DR InterPro; IPR048356; MS_N.
DR InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR NCBIfam; TIGR01344; malate_syn_A; 1.
DR PANTHER; PTHR42902; MALATE SYNTHASE; 1.
DR PANTHER; PTHR42902:SF1; MALATE SYNTHASE 1-RELATED; 1.
DR Pfam; PF20659; MS_C; 1.
DR Pfam; PF20656; MS_N; 1.
DR Pfam; PF01274; MS_TIM-barrel; 1.
DR PIRSF; PIRSF001363; Malate_synth; 1.
DR SUPFAM; SSF51645; Malate synthase G; 1.
DR PROSITE; PS00510; MALATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Glyoxylate bypass {ECO:0000256|ARBA:ARBA00022435,
KW ECO:0000256|RuleBase:RU000555};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000555};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW ECO:0000256|RuleBase:RU000555}.
FT DOMAIN 6..65
FT /note="Malate synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF20656"
FT DOMAIN 156..401
FT /note="Malate synthase TIM barrel"
FT /evidence="ECO:0000259|Pfam:PF01274"
FT DOMAIN 406..524
FT /note="Malate synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20659"
FT ACT_SITE 160
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
FT ACT_SITE 440
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
SQ SEQUENCE 526 AA; 60093 MW; B529DF981F4BF356 CRC64;
MAEDIEIKGT ISPGFSEILT TEALRFVANL ARKFEATRSK LLKAREIRQN EINQGKMPDF
LRETKSIRES DWNVAPIPKD LLERKVEITG PVERKMIVNA LNSGAHVFMA DFEDANSPTW
ENNIQGHINL RDAVNRKIDF VSPEGKAYKL APKTATLVVR PRGWHLNEKH MLVDGQIVSA
SLFDFGLYFF HNAHALIKNG TGPYFYLPKM ESHLEARLWN DVFNFSQDQL RVPKGTIRAT
VLIETILAAF EMDEILYELK DHSSGLNCGR WDYIFSFIKK FREKREFLLP DRAQVTMEKA
FLKAYVELLI KTCHRRGIHA MGGMAAQIPI KSDAAANEAA LEKVRQDKLR EVKAGHDGTW
VAHPGLVEVA MDIFNQHMKG PNQLQVKREE VAITAKDLLQ VPNGDITEKG LRININVGIL
YIEAWLRGQG CVPLYNLMED AATAEISRSQ VWQWIRHEAK MINGTLITKE LVKKMIQEEL
ETIRKTIGPE QYNAGEFKRA SELFEHISTK NEYPEFLTLV AYDYID
//
