ID A0A1G1LK58_9BACT Unreviewed; 450 AA.
AC A0A1G1LK58;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE SubName: Full=B12-binding domain-containing radical SAM protein {ECO:0000313|EMBL:OGX05180.1};
GN ORFNames=A3G87_06730 {ECO:0000313|EMBL:OGX05180.1};
OS Omnitrophica bacterium RIFCSPLOWO2_12_FULL_50_11.
OC Bacteria; Candidatus Omnitrophota.
OX NCBI_TaxID=1801834 {ECO:0000313|EMBL:OGX05180.1, ECO:0000313|Proteomes:UP000177832};
RN [1] {ECO:0000313|EMBL:OGX05180.1, ECO:0000313|Proteomes:UP000177832}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGX05180.1}.
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DR EMBL; MHFT01000055; OGX05180.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G1LK58; -.
DR STRING; 1801834.A3G87_06730; -.
DR Proteomes; UP000177832; Unassembled WGS sequence.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR025274; DUF4070.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF12; BLL1977 PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF13282; DUF4070; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485}.
FT DOMAIN 18..147
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 172..391
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 450 AA; 51443 MW; EF28A702419F8C41 CRC64;
MKIGLIAMSG VRAYNEELLR FGLTLPGFVE RSKVIASLPS LSLLTLAALT APEVQVEYWE
IKDLIDQDGL PTDFDLVAIS SFSAQIYDAY QIADRCRKRG IAVVMGGLHV TSLPEEAREH
CTSIVVGEGE ELWPVVVEDF LNDRLQDSYV QPPGHTFELA NSPIPRYELL EVSKYNRLTV
QTSRGCPHRC DFCASSILLT SKYCVKPVHK VIAEIRKIKE IWKQPFIEFA DDNSFVLRHH
YKELLRQLVG ERISWFTEAD ISIAEDRDLL HLMRESGCRQ VLIGLESPRG AGLDGIELKR
NWKLKKQPDY EAAIKIIQSH GITVNGCFIL GLDGDTEEVF DSIYRFVERT GLYDVQITVL
TPFPGTPLYD RFKREGRILE DQAWHKCTLF DVNYYPKLMT VETLEKGLVD LAKKIYATSF
IRERRKRFFR ILRQEGLMLA AQADFQKEGT
//
