ID A0A1G1NNM0_9BACT Unreviewed; 421 AA.
AC A0A1G1NNM0;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=Methyltransferase {ECO:0000256|RuleBase:RU362026};
DE EC=2.1.1.- {ECO:0000256|RuleBase:RU362026};
GN ORFNames=A3D27_03700 {ECO:0000313|EMBL:OGX31255.1};
OS Omnitrophica WOR_2 bacterium RIFCSPHIGHO2_02_FULL_46_37.
OC Bacteria; Candidatus Omnitrophota.
OX NCBI_TaxID=1801855 {ECO:0000313|EMBL:OGX31255.1, ECO:0000313|Proteomes:UP000179974};
RN [1] {ECO:0000313|EMBL:OGX31255.1, ECO:0000313|Proteomes:UP000179974}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-
CC methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:16857, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:13674,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:85452, ChEBI:CHEBI:137933; EC=2.1.1.113;
CC Evidence={ECO:0000256|ARBA:ARBA00001893};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family. N(4)
CC subfamily. {ECO:0000256|ARBA:ARBA00010203}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGX31255.1}.
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DR EMBL; MHGO01000048; OGX31255.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G1NNM0; -.
DR Proteomes; UP000179974; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0015667; F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 2.40.50.90; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR002941; DNA_methylase_N4/N6.
DR InterPro; IPR017985; MeTrfase_CN4_CS.
DR InterPro; IPR001091; RM_Methyltransferase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR035437; SNase_OB-fold_sf.
DR InterPro; IPR016071; Staphylococal_nuclease_OB-fold.
DR PANTHER; PTHR13370:SF32; DNA ADENINE METHYLTRANSFERASE YHDJ; 1.
DR PANTHER; PTHR13370; RNA METHYLASE-RELATED; 1.
DR Pfam; PF01555; N6_N4_Mtase; 1.
DR Pfam; PF00565; SNase; 1.
DR PRINTS; PR00508; S21N4MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR SUPFAM; SSF50199; Staphylococcal nuclease; 1.
DR PROSITE; PS00093; N4_MTASE; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:OGX31255.1};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 9..239
FT /note="DNA methylase N-4/N-6"
FT /evidence="ECO:0000259|Pfam:PF01555"
FT DOMAIN 343..413
FT /note="TNase-like"
FT /evidence="ECO:0000259|Pfam:PF00565"
SQ SEQUENCE 421 AA; 48458 MW; D5116B9C2E1D0CCA CRC64;
MQEVKDASVH LVVTSPPYWQ LKDYGSSDQI GFNHSYEEYI NNLNLVWNEC YRVIENGCRL
CINIGDQFAR SVYYGRYKVI PIRTEIIKFC ETIGFDYMGA VIWQKVTTCN TTGGASVMGS
FPYPRNGILK LDYEFILIFK KQGVASPVTH EIREKSKMTT EEWNQYFSGH WNFAGEKQGK
HLAMFPEELP KRLIKMFTFV GDTVLDPFLG SGTTTLAAKN LGRNSVGYEI NGDFLTFIKE
RVGCGQSLFS EECAFEISKQ EKIIANFAEQ IQELPYIFED PIKVDKKIDP KKLQFGSKID
QDSGQREEYF TVKEVMSPEL IKLSNGLTVR LLGIKQDVLK NGKATEYLVN KTQGQKVFLK
YDQTKYDSEN RLLCYLYLKN KTFVNAHLIK EGLAVVDADM LFKYKEKFLE LQNLEHAHNE
K
//
