ID A0A1G1WKU3_9BACT Unreviewed; 630 AA.
AC A0A1G1WKU3;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
DE Flags: Fragment;
GN ORFNames=A2Z42_00110 {ECO:0000313|EMBL:OGY28000.1};
OS Candidatus Woykebacteria bacterium RBG_19FT_COMBO_43_10.
OC Bacteria; Candidatus Woykebacteria.
OX NCBI_TaxID=1802598 {ECO:0000313|EMBL:OGY28000.1, ECO:0000313|Proteomes:UP000176645};
RN [1] {ECO:0000313|EMBL:OGY28000.1, ECO:0000313|Proteomes:UP000176645}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrB family.
CC {ECO:0000256|ARBA:ARBA00010708}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGY28000.1}.
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DR EMBL; MHCU01000016; OGY28000.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G1WKU3; -.
DR Proteomes; UP000176645; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd16928; HATPase_GyrB-like; 1.
DR CDD; cd00822; TopoII_Trans_DNA_gyrase; 1.
DR CDD; cd03366; TOPRIM_TopoIIA_GyrB; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.670; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR InterPro; IPR002288; DNA_gyrase_B_C.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR001241; Topo_IIA.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR000565; Topo_IIA_B.
DR InterPro; IPR013759; Topo_IIA_B_C.
DR InterPro; IPR013506; Topo_IIA_bsu_dom2.
DR InterPro; IPR018522; TopoIIA_CS.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR034160; TOPRIM_GyrB.
DR PANTHER; PTHR45866:SF1; DNA GYRASE SUBUNIT B, MITOCHONDRIAL; 1.
DR PANTHER; PTHR45866; DNA GYRASE/TOPOISOMERASE SUBUNIT B; 1.
DR Pfam; PF00204; DNA_gyraseB; 1.
DR Pfam; PF00986; DNA_gyraseB_C; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR01159; DNAGYRASEB.
DR PRINTS; PR00418; TPI2FAMILY.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00433; TOP2c; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
DR PROSITE; PS00177; TOPOISOMERASE_II; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:OGY28000.1};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 412..526
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:OGY28000.1"
SQ SEQUENCE 630 AA; 69846 MW; B6B9324484FA9E0E CRC64;
PVRRRPGMYI GSTDVHGLHH LVAEIVDNSV DEALAGYAKN IWVVIHNDEK VTIIDDGRGI
PVEEHQKTGK SALEVTMTYL HAGGKFGGQG YKVSGGLHGV GASAVNALSE DMRVEVKRGG
KIYTQAYKVG KPVTKVTSST DSQLEPNAQK IWSKIHNGTA TTFKPDKRIF STISPEFKIL
EKQLRERAYL VAGLFFHIRD ERTDTEENFY FDGGIKSLVE DLNKNKNTYH IPIFIEKDAE
NMAVEVAVQY NDSFTETVES FANVINTTEG GTHLTGFRIA LTRAINDYSR KIGATKDIEG
GLSGEDIREG LTAVVAIKMN SEKLEFEGQT KTKLGNSEVQ PVVQSVVKEG LDVFFEENPS
EARKIIEKVL LAARARLAAK AAKEAVIRKG ALEGMTLPGK LADCQEKDPS ASEIYIVEGD
SAGGSAKQGR DRRFQAILPL GGKILNTERA RLDKIISFEE IKDLIIALGA GIADTFNIDK
IRYHRVILMT DADVDGEHIR TLLLTFFFRY MPAVLEKGYL YIAQPPLYKI TSGKEFNYAY
NDGEKDALVK GDGRRNAKAS LSIQRYKGLG EMNPEQLWET TMNPATRTLK KVNMEDATTA
DKVFNMLMGQ EVPPRKKFIQ THAKYATLDV
//
