ID A0A1G1YDL0_9BACT Unreviewed; 336 AA.
AC A0A1G1YDL0;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 08-NOV-2023, entry version 29.
DE RecName: Full=dTDP-glucose 4,6-dehydratase {ECO:0000256|ARBA:ARBA00011990, ECO:0000256|RuleBase:RU004473};
DE EC=4.2.1.46 {ECO:0000256|ARBA:ARBA00011990, ECO:0000256|RuleBase:RU004473};
GN ORFNames=A3J59_04185 {ECO:0000313|EMBL:OGY50314.1};
OS Candidatus Buchananbacteria bacterium RIFCSPHIGHO2_02_FULL_56_16.
OC Bacteria; Candidatus Buchananbacteria.
OX NCBI_TaxID=1797542 {ECO:0000313|EMBL:OGY50314.1, ECO:0000313|Proteomes:UP000177310};
RN [1] {ECO:0000313|EMBL:OGY50314.1, ECO:0000313|Proteomes:UP000177310}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose
CC + H2O; Xref=Rhea:RHEA:17221, ChEBI:CHEBI:15377, ChEBI:CHEBI:57477,
CC ChEBI:CHEBI:57649; EC=4.2.1.46;
CC Evidence={ECO:0000256|ARBA:ARBA00001539,
CC ECO:0000256|RuleBase:RU004473};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|ARBA:ARBA00001911,
CC ECO:0000256|RuleBase:RU004473};
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. dTDP-glucose dehydratase subfamily.
CC {ECO:0000256|ARBA:ARBA00008178, ECO:0000256|RuleBase:RU004473}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGY50314.1}.
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DR EMBL; MHIL01000032; OGY50314.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G1YDL0; -.
DR STRING; 1797542.A3J59_04185; -.
DR Proteomes; UP000177310; Unassembled WGS sequence.
DR GO; GO:0008460; F:dTDP-glucose 4,6-dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0009225; P:nucleotide-sugar metabolic process; IEA:InterPro.
DR CDD; cd05246; dTDP_GD_SDR_e; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR Gene3D; 3.90.25.10; UDP-galactose 4-epimerase, domain 1; 1.
DR InterPro; IPR005888; dTDP_Gluc_deHydtase.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR NCBIfam; TIGR01181; dTDP_gluc_dehyt; 1.
DR PANTHER; PTHR43000:SF7; DTDP-D-GLUCOSE 4,6-DEHYDRATASE; 1.
DR PANTHER; PTHR43000; DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED; 1.
DR Pfam; PF16363; GDP_Man_Dehyd; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|RuleBase:RU004473}; NAD {ECO:0000256|ARBA:ARBA00023027}.
FT DOMAIN 5..307
FT /note="NAD(P)-binding"
FT /evidence="ECO:0000259|Pfam:PF16363"
SQ SEQUENCE 336 AA; 38356 MW; FCF64BAC583637BD CRC64;
MSKILVTGGA GFIGSNFIRY WLSRHPGDEM INLDKLTYAG NLENLKDAEG KPGYRFVLGD
ITNVDIVRDL VRQCDLIVHF AAESHVDRSI IDAGEFIKTN VVGTQLLLQA AKDFDKRMHH
VSTDEVYGAL SLETKKRFNE ASPFNPTSPY AASKAASDHL VMAYHHTHGL SVTISNCSNN
FGPYQFPEKF IPLAITNLLE GKKVPVYGDG LYVRDWLYVD DHCRAIDLIL EKGQTGQTYC
IGGMTKSVNN LAVIKKILKL LGQGEEMIEF VKDRPGHDRR YAVDWSRAKK ELGYRPEHDF
DEYLKQTVEW YQKNEPWWQN VKSGEYREYY QTQYGK
//
