ID A0A1G2FXR7_9BACT Unreviewed; 599 AA.
AC A0A1G2FXR7;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=A2W41_00020 {ECO:0000313|EMBL:OGZ42512.1};
OS Candidatus Ryanbacteria bacterium RIFCSPHIGHO2_01_45_13.
OC Bacteria; Candidatus Ryanbacteria.
OX NCBI_TaxID=1802112 {ECO:0000313|EMBL:OGZ42512.1, ECO:0000313|Proteomes:UP000176700};
RN [1] {ECO:0000313|EMBL:OGZ42512.1, ECO:0000313|Proteomes:UP000176700}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGZ42512.1}.
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DR EMBL; MHNI01000017; OGZ42512.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G2FXR7; -.
DR Proteomes; UP000176700; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.30.160.800; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 7..283
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 284..512
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 28..35
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 599 AA; 68288 MW; 070430C9A66DEC98 CRC64;
MTNTTSENLN PRQKEAVEYG NGPILIAAGA GSGKTKTLTA RLANIIKTGA TPESIVAITF
TNKAAKEMRE RIENCKIPAC RQAGKIGNLF VGTFHSFGAR ILKKEAKLAG RMSYFTIFDS
DDSLSLIKKI TKALDISRDQ YNPMALQSKI SQMKDEFLDP SEFDRTAALV FKKYEDELVK
NNAFDFDDLI EKPVRIFQNH PEVLAKYQKQ FRYILVDEYQ DINTAQYRLI RLLAENHRNL
SVVGDDQQAI YSFRGADFRN FLNFARDWPD AKIIKLEQNY RSTGTIISAA SQLISNNKLQ
TPKTLWTENP AGNSIKVVKA PDAETEASWI ASEIARPPVR RENCAVLYRT NAQSRAVEQA
LIFANLPYKI FGGLKFYERK EIKDLMSGLR LAHNPKDTIS EERIKKNFNK KIAAVLIDEL
PRLGKNLGVL ELINFFIVNA DYLEYLEKNY NNYQERIENV NELIHFASEF KTLEEFLERA
ALLQSADNPK NSPLFAYNSP PVNLMSIHLS KGLEFDSVFL VGCSEGLMPH QMSYGSLDEL
EEERRLMYVA MTRARQKLYL SFSGLPSRFL YEVPPELTDF EDLDGGRREL PSEEETYIE
//
