ID A0A1G2HVE9_9BACT Unreviewed; 1319 AA.
AC A0A1G2HVE9;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
GN ORFNames=A2822_01635 {ECO:0000313|EMBL:OGZ66435.1};
OS Candidatus Staskawiczbacteria bacterium RIFCSPHIGHO2_01_FULL_41_41.
OC Bacteria; Candidatus Staskawiczbacteria.
OX NCBI_TaxID=1802203 {ECO:0000313|EMBL:OGZ66435.1, ECO:0000313|Proteomes:UP000178774};
RN [1] {ECO:0000313|EMBL:OGZ66435.1, ECO:0000313|Proteomes:UP000178774}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrA/ParC subunit
CC family. {ECO:0000256|ARBA:ARBA00008263}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OGZ66435.1}.
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DR EMBL; MHOP01000005; OGZ66435.1; -; Genomic_DNA.
DR Proteomes; UP000178774; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:UniProtKB-EC.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd00187; TOP4c; 1.
DR Gene3D; 3.30.1360.40; -; 1.
DR Gene3D; 2.120.10.90; DNA gyrase/topoisomerase IV, subunit A, C-terminal; 1.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR Gene3D; 3.90.199.10; Topoisomerase II, domain 5; 2.
DR Gene3D; 1.10.268.10; Topoisomerase, domain 3; 1.
DR InterPro; IPR006691; GyrA/parC_rep.
DR InterPro; IPR035516; Gyrase/topoIV_suA_C.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR013758; Topo_IIA_A/C_ab.
DR InterPro; IPR013757; Topo_IIA_A_a_sf.
DR InterPro; IPR002205; Topo_IIA_dom_A.
DR NCBIfam; TIGR01063; gyrA; 1.
DR NCBIfam; TIGR01443; intein_Cterm; 1.
DR PANTHER; PTHR43493:SF5; DNA GYRASE SUBUNIT A, CHLOROPLASTIC_MITOCHONDRIAL; 1.
DR PANTHER; PTHR43493; DNA GYRASE/TOPOISOMERASE SUBUNIT A; 1.
DR Pfam; PF03989; DNA_gyraseA_C; 6.
DR Pfam; PF00521; DNA_topoisoIV; 2.
DR Pfam; PF14890; Intein_splicing; 1.
DR Pfam; PF14528; LAGLIDADG_3; 2.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00434; TOP4c; 1.
DR SUPFAM; SSF101904; GyrA/ParC C-terminal domain-like; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF55608; Homing endonucleases; 2.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 2.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 218..365
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT DOMAIN 542..564
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..23
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1319 AA; 146655 MW; 062AC929D339977B CRC64;
MAKDKEKDPA KSPKGDRGAA KKEEQATNAI GNVSKREIVD ELKESYIDYA MSVIVARALP
DVRDGLKPVH RKILYTMNEM GLNASAKFRK SATVVGECFV KDTLILSKKG LVSIQDIQVG
DEVYTQKGMQ KVVQTYEMPE KPLLKVVLDN GLSNVVTLSQ KFKVLTPDWK FIWKEAKDLT
DQDYLIIKSD YPEIGHQVKL QTTGEDQPSH LNENVAYILG LFVSDGWISE DYSAKKTTRI
GFTAGNDKEI AERIVSIFKK EFNHHPSIEE RLFDGRRKKI YNVRVNSAAI NEFFVANFDI
KGAWALTKKI PNEIFQSPSD IISSFISGLI EGDGYISHKQ NRVNYVSISE KLTDQLMALL
LSQGIFSSKY KTDVVSGDSA VLNRKINGKH PSFSLEIKGE NAVALSSKLN LVSKSKGQRA
ARLGSKKLTS HDRKGPNRHD VIPYASNILF QELSNNHLGG GWYQDVDGEK FRMGVKYPDG
TKIRYSSNLH EMPLRKTQVI DWGIKDKLEK IGSPNLEFLE EIIKNKIHFL KVSLIKKVAP
QKTFDFEVEN DHEFIANGVV SHNCMGKYHP HGDMAIYDSL VRMAQDFNMR YPLAHPQGNF
GSQDGDSAAA PRYTEIKLSK IGELLLADIE KDTVRFMDNY DGTRQEPTVL PSPVPQLLLN
GSLGIAVGMA TNIPPHNLTE LTDAISHILD NPKAETPDLF QFIQGPDFPT GGIIYDQKEI
ITAYSQGKGS IVMRGKAEIT EKKDGSEQIL ITEIPYQVLK STLVEEMANL VSEKKIEGIR
DIKDLSDREG MGIIIDIKKG FDANRVLNRL YKFTNLQKTF HLNLLALVDG IQPEILSIAD
VLKYFISHRT EVVTRRTKFD LEKAKDRAHI LEGLMIALNN IDAVIKVIKQ SKDREDAKLN
LIKKFKLSEK QAVAILEMKL QTLAGLERQK IQDELKAILE LIKELTAILA SPAKLKAIIK
KELAEVKEKF GDARRTKVVK GKLGEITEID LVPLEETIVT LTTGGYIKRI NPATYKIQKR
GGKGIIGMKT MQEDIVEHFL SASTHDNLMF FTDSGKVFQT QVYEIPEGTR VARGRGISNF
LEGISNEEKV LSLVTKAKAK PDAPSADKYL VMVTKNGRIK KTSLEEFENV RKSGIIAIKL
EKGDALKKVV KTSGEDDIVL ATKSGIAIRF KEKDIRPMGR SAAGVKGIRI KGNDEVIGMD
VVTNEAASAK GSGAPKQYLM VVMENGYGKR TEISEYKVQG RGGSGIKTAN ITAKTGGIVI
SSILHDSVDE EDLIVISQHG QVIRTATKSI SVLGRATQGV RIMRLEPGDK VASGTCVKE
//
