ID A0A1G2UT42_9BACT Unreviewed; 985 AA.
AC A0A1G2UT42;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=A3G99_01940 {ECO:0000313|EMBL:OHB12559.1};
OS Candidatus Zambryskibacteria bacterium RIFCSPLOWO2_12_FULL_39_23.
OC Bacteria; Candidatus Zambryskibacteria.
OX NCBI_TaxID=1802776 {ECO:0000313|EMBL:OHB12559.1, ECO:0000313|Proteomes:UP000176558};
RN [1] {ECO:0000313|EMBL:OHB12559.1, ECO:0000313|Proteomes:UP000176558}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OHB12559.1}.
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DR EMBL; MHWT01000014; OHB12559.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G2UT42; -.
DR Proteomes; UP000176558; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 15..335
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 336..600
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 36..43
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 985 AA; 114179 MW; C763E5F57D88D8FC CRC64;
MVRTKFDEGF EERYKALNKE QKEAVDTIEG PVLVVAGPGT GKTEILTLRI ANILKKTDTK
PENILALTFT DAASGNMRRR LISLIEASAY RVNIETFHSF CNKIIKDYPE YFPTIIGSSN
ITEVESVDIL EGLIEKLLLQ ILKPWGDPFY YIRDIYKKIE ELKREGISPE KFTELLAKEE
RRFKSRDDLI HEKGAHKGKM KSEHKDFERQ LEKNKELAMV YAAYQKILGE KRLYDWSDMI
MEVLQGIEKY PDLKLNLQEN HQYILVDEHQ DTNNAQNKIL EMMGDFHKNP NIFIVGDEKQ
AIFRFQGASI ENFNYFKKLY PKAKLVRLGI NYRSGQNILD AAHSLLSPGG QLISSDKKKV
SNIKVGEFTD KNVELYFIAE KIKELLKKNV SPKEITILYR SNKEAFPLAE VLEKSGIIYS
IESDENLLGE RFVRKILAIL LAIENFENDQ FLVPVLHIEE FDIDPVDAFR LINEAGRKRQ
SIYVLMSKSG IPPIKKLFGK LKEWVRHSKN DHLTEFLERI LRESGLLDSM IRSRDAEAFL
GIEKFFEEGK RISANMRGAG FSDFMRYISI IQKRKIFIKK PKHNIREDAI RLMTTHRSKG
LEFEYVFIIN AGERSFGSKA DRDALRLIPS IYSLGNAVSK LDTGTEEDER RLFYVAMTRA
KFEAYITYSS FDENGKEILP SPFILEIRED RKEKLKTQQF EKNLKSDPKK LYAERQKTGG
REIDRKFVTE LFNSHPLSVT ALNNYLTCPW KYFYRNLLRI PTAPEKHQIY GIAMHNAVED
LWKAMKDREV DKKFLLSSYK RHLGLLGVLR QSEFEEALKR GEKALSGWLL WANPKINNPV
VSEFSIKGAE LSSGIVLSGK LDKVEFVSPK KAIVTDYKTG SPKTRNFIEG KTKERTGDIK
RQLVFYKLLL ILNDDLEMTS GVVEFLEPNT SGKYNKEEFE IKESEIKELK QIILKFAEEI
KSLSFWDKTC RDKNCEYCSL RKLLK
//
