ID A0A1G3CQ65_9BURK Unreviewed; 1184 AA.
AC A0A1G3CQ65;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 24-JAN-2024, entry version 30.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=A2X74_08855 {ECO:0000313|EMBL:OHC09618.1};
OS Polynucleobacter sp. GWA2_45_21.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Polynucleobacter.
OX NCBI_TaxID=1801989 {ECO:0000313|EMBL:OHC09618.1, ECO:0000313|Proteomes:UP000176957};
RN [1] {ECO:0000313|EMBL:OHC09618.1, ECO:0000313|Proteomes:UP000176957}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OHC09618.1}.
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DR EMBL; MHZG01000008; OHC09618.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G3CQ65; -.
DR STRING; 1801989.A2X74_08855; -.
DR Proteomes; UP000176957; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}.
FT DOMAIN 642..803
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 824..978
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT COILED 1148..1175
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1184 AA; 132554 MW; AAC72672CC477C84 CRC64;
MSDALKLAPP IPAPRAGQRF TFSGLVGSSD AALIAQSALR YRSEFSVMVI FCAQAQEAQR
LLEEIPAFAP QLKTRLLPDW EILPYDHFSP HQDLVSERLA TLYELLNGSC DIVLVPITTA
LQRLGPPNFL SGHTFFFRQG DKLNEAALKL QLQQAGYDPV SSVMRPGEYS IRGGLIDLFP
MGSSLPYRLD LFGDEIEQIR AFDPDTQRSL YPVKGVRLLP GHEFPFDDAS RTAFRGRWRE
VFEGDPTRCS IYKDANLGIP SAGIESYLPL FFEESSTVFD YFPRSGDPVW LVSIGDVEES
IKSFWKDTLS RYEFLKHDLD RPILPPADLF LDVDQFFTAL KPNARLALEK EVDKEAKESP
QFLAVPDLAV HRRDADPINR LRALVAQEKV RVLICSDSNG RKESIRQLFE ESNSVAGQNG
KPLYPLKPEG FEGIADFIKS DSLFGLVTAQ LFNGFTWPAE NLIVVTEAEL FTTTARQRRK
GKESESADPD MLFKDLSELK IGDPVVHSDH GIGRYQGLVL LNLAPPKEEP IFEEFLHLVY
AKDATLYVPV QQLQMVTRYA GSDPDSAPLH QLGSGQWDKA RRKAAQQIRD TAAELLSLYA
ARAIRKGHAF EFSAHDYAAF AESFGFEETP DQANAIAAVI GDMTSGTPMD RLVCGDVGFG
KTEVALRASF VAVMGGKQVA ILAPTTLLAE QHVATWKDRF ADWPVRIVEL SRFKTTKEIN
AALEAIAKGE ADIIIGTHKL LSKETQFANL GLVIADEEHR FGVRQKDALK ALRAEVDILT
LTATPIPRTL GMAMEGLREF SIIATAPQKR LAIKTFVRRE GDGVIREAVL REIKRGGQVY
FLHNEVETIQ NRKHALQELI PEARISVAHG QMHERELESV MREFVTQRTN ILLCTTIIET
GIDVPTANTI IMHRADKFGL AQLHQLRGRV GRSHHQAYAY LLVPDPEALS KQAQLRLNAI
QAMEELGSGF YLAMHDLEIR GAGEVLGDKQ SGEIHEIGFQ LYTEMLNRAV KSLRSGKEPD
LLSPLQATTD VNLGVPALFP NDYCPDVHER LSMYKRFAGT HDFSELMGLR EELVDRYGDL
PDQAKSLYET HRLRLEMAGF GIKKIDASPS SIQIQFIPNP PIDPMKIIHL IQSSKYIQLN
GQDKLKILPQ KEKDFEKLEQ RLDQIRKILR SLNESAVLNT AQVN
//
