ID A0A1G3QAL4_9SPIR Unreviewed; 471 AA.
AC A0A1G3QAL4;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 27-MAR-2024, entry version 19.
DE SubName: Full=Radical SAM protein {ECO:0000313|EMBL:OHD63754.1};
GN ORFNames=A2176_14550 {ECO:0000313|EMBL:OHD63754.1};
OS Spirochaetes bacterium RBG_13_51_14.
OC Bacteria; Spirochaetota.
OX NCBI_TaxID=1802193 {ECO:0000313|EMBL:OHD63754.1, ECO:0000313|Proteomes:UP000176595};
RN [1] {ECO:0000313|EMBL:OHD63754.1, ECO:0000313|Proteomes:UP000176595}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=27774985; DOI=10.1038/ncomms13219;
RA Anantharaman K., Brown C.T., Hug L.A., Sharon I., Castelle C.J.,
RA Probst A.J., Thomas B.C., Singh A., Wilkins M.J., Karaoz U., Brodie E.L.,
RA Williams K.H., Hubbard S.S., Banfield J.F.;
RT "Thousands of microbial genomes shed light on interconnected biogeochemical
RT processes in an aquifer system.";
RL Nat. Commun. 7:13219-13219(2016).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OHD63754.1}.
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DR EMBL; MIBH01000062; OHD63754.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G3QAL4; -.
DR STRING; 1802193.A2176_14550; -.
DR Proteomes; UP000176595; Unassembled WGS sequence.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR036724; Cobalamin-bd_sf.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF12; BLL1977 PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF52242; Cobalamin (vitamin B12)-binding domain; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS01278; MTTASE_RADICAL; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022485}.
FT DOMAIN 1..132
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 169..388
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 471 AA; 54478 MW; 49707E5BFFBA8962 CRC64;
MGTVIFIEPK PSGEHIFSRF ALPRIGIFLL ATMMKRRGWH TEVIIEEFQK IDYKVLSDAD
IIGISTITPT AVNAYTIADT VRSMGIPVIM GGPHVTYLPD EALVHADYVI RGEGETPLMR
FIDAWEHQSD YYAVPNLSFR DGGRIIHNPM MNLDMNLDQF PLCDFSLIQG GGELRVIPIQ
TSRGCPQDCS FCSVTGMFGR KYRYRSTQHI LDELRYYKDS GKMIFFYDDH FAADRKRAKE
LLRAMLHEGL HFKWSTQARA DIVYDLELIE LMKQSGCHTL FIGFESFNPK SLKEMNKKQT
VDDIFNAARI IQSFNINIHG MFVHGFDHDT WESVKHTVRL AKKMGITSAQ FLVLTPFPGS
TFSDSIADRI VFKEWSMYDA HHVVYQPLHF TLRGLQRAQT YSHENFYSAV EIVKKLFRRK
WLDCGIALYA RFINWKWKKN NRRYLRLISD ISREQAPMPT EIHAVEDSIT A
//
