ID A0A1G4FKS8_9FIRM Unreviewed; 639 AA.
AC A0A1G4FKS8;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 24-JAN-2024, entry version 26.
DE RecName: Full=Aconitate hydratase A {ECO:0000256|ARBA:ARBA00019378};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
DE AltName: Full=Iron-responsive protein-like {ECO:0000256|ARBA:ARBA00031977};
DE AltName: Full=RNA-binding protein {ECO:0000256|ARBA:ARBA00031081};
GN Name=dmdA {ECO:0000313|EMBL:SCL89626.1};
GN ORFNames=PP176A_1817 {ECO:0000313|EMBL:SCL89626.1};
OS Sporanaerobacter sp. PP17-6a.
OC Bacteria; Bacillota; Tissierellia; Tissierellales; Sporanaerobacteraceae;
OC Sporanaerobacter.
OX NCBI_TaxID=1891289 {ECO:0000313|EMBL:SCL89626.1, ECO:0000313|Proteomes:UP000179042};
RN [1] {ECO:0000313|EMBL:SCL89626.1, ECO:0000313|Proteomes:UP000179042}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PP176A {ECO:0000313|EMBL:SCL89626.1};
RA Seilhamer J.J.;
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FMIF01000017; SCL89626.1; -; Genomic_DNA.
DR RefSeq; WP_071140113.1; NZ_FMIF01000017.1.
DR AlphaFoldDB; A0A1G4FKS8; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000179042; Unassembled WGS sequence.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd01585; AcnA_Bact; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR006250; Aconitase_put.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR01342; acon_putative; 1.
DR PANTHER; PTHR43160; ACONITATE HYDRATASE B; 1.
DR PANTHER; PTHR43160:SF3; ACONITATE HYDRATASE, MITOCHONDRIAL; 1.
DR Pfam; PF00330; Aconitase; 2.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Lyase {ECO:0000313|EMBL:SCL89626.1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000179042}.
FT DOMAIN 7..284
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 283..407
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 516..575
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 639 AA; 69704 MW; 9B0934463A329818 CRC64;
MRLTVAQKII KDHLSIGEMK KGTEIGIRID QTLTQDSTGT MAYLQLESLG VDKVKTKRSV
AYIDHNMLQT GPENADDHRY IQSVAKKYGI YFSKPGNGIC HQVHLERFGV PGETLLGSDS
HTPTCGGIGM IAIGAGGLDV AVAMAGGEYF ITMPEIVKVE LKGCLKPGVS AKDIILEILR
RYKVKGGVNK IFEYWGEGVK SLTVPERATI TNMGAELGAT TSVFPSDEVT YEFLKAQGRG
SDYREITADE GAVFDEEFVI NLDELRPLVA RYHSPDNVVP IGKVKNIRVD QVAIGSCTNS
SYRDLMRVAQ ILDGKTVAEH VSLTISPGSK QVLNMLAENG ALSKIISSGA RILECGCGPC
IGMGQAPNTD GVSLRTFNRN FKGRSGTPSA KVYLASPEVA AVSALTGYIT DPTKYISEGD
INLPKHFLIN DNSIIPPAEK GENVEIVKGP NIKPFPQGKP LTRDIGGKVL IKLGDNITTD
HIMPSNAKLL PYRSNIPYLA DYCLLPCDSE FPKKVKKFGG GIIVSGFNYG QGSSREHAAL
VPLYLGVKAV LAKSFARIHK QNLINNGIIP LTFRQEKDYD DIDVMDELVL ENILEQMEGD
TIVANNVTQN KRYTLILDET ERQREILRAG GLLNFIKSR
//
