ID A0A1G4JIV6_9SACH Unreviewed; 815 AA.
AC A0A1G4JIV6;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Ribosome-releasing factor 2, mitochondrial {ECO:0000256|HAMAP-Rule:MF_03059};
DE Short=RRF2mt {ECO:0000256|HAMAP-Rule:MF_03059};
DE AltName: Full=Elongation factor G 2, mitochondrial {ECO:0000256|HAMAP-Rule:MF_03059};
DE Short=EF-G2mt {ECO:0000256|HAMAP-Rule:MF_03059};
DE Short=mEF-G 2 {ECO:0000256|HAMAP-Rule:MF_03059};
GN Name=MEF2 {ECO:0000256|HAMAP-Rule:MF_03059};
GN ORFNames=LANO_0D08636G {ECO:0000313|EMBL:SCU90408.1};
OS Lachancea nothofagi CBS 11611.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Lachancea.
OX NCBI_TaxID=1266666 {ECO:0000313|EMBL:SCU90408.1};
RN [1] {ECO:0000313|EMBL:SCU90408.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Ploux O.;
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mitochondrial GTPase that mediates the disassembly of
CC ribosomes from messenger RNA at the termination of mitochondrial
CC protein biosynthesis. Not involved in the GTP-dependent ribosomal
CC translocation step during translation elongation. {ECO:0000256|HAMAP-
CC Rule:MF_03059}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000256|HAMAP-Rule:MF_03059}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. EF-G/EF-2
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_03059}.
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DR EMBL; LT598448; SCU90408.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G4JIV6; -.
DR OrthoDB; 148165at2759; -.
DR Proteomes; UP000189911; Chromosome d.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0032543; P:mitochondrial translation; IEA:UniProtKB-UniRule.
DR GO; GO:0032790; P:ribosome disassembly; IEA:UniProtKB-UniRule.
DR CDD; cd01886; EF-G; 1.
DR CDD; cd16262; EFG_III; 1.
DR CDD; cd03713; EFG_mtEFG_C; 1.
DR Gene3D; 3.30.70.240; -; 1.
DR Gene3D; 3.30.70.870; Elongation Factor G (Translational Gtpase), domain 3; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR HAMAP; MF_03059; mEF_G_2; 1.
DR InterPro; IPR030851; EFG2.
DR InterPro; IPR041095; EFG_II.
DR InterPro; IPR009022; EFG_III.
DR InterPro; IPR035647; EFG_III/V.
DR InterPro; IPR035649; EFG_V.
DR InterPro; IPR000640; EFG_V-like.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR031157; G_TR_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR43261:SF1; RIBOSOME-RELEASING FACTOR 2, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43261; TRANSLATION ELONGATION FACTOR G-RELATED; 1.
DR Pfam; PF00679; EFG_C; 1.
DR Pfam; PF14492; EFG_III; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SMART; SM00838; EFG_C; 1.
DR SUPFAM; SSF54980; EF-G C-terminal domain-like; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR PROSITE; PS00301; G_TR_1; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 3: Inferred from homology;
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134, ECO:0000256|HAMAP-
KW Rule:MF_03059};
KW Mitochondrion {ECO:0000256|ARBA:ARBA00023128, ECO:0000256|HAMAP-
KW Rule:MF_03059};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_03059}; Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_03059}.
FT DOMAIN 41..327
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
FT BINDING 50..57
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_03059"
FT BINDING 115..119
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_03059"
FT BINDING 167..170
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_03059"
SQ SEQUENCE 815 AA; 90003 MW; 7E5DEF853706BBAB CRC64;
MNLKVSASKA FGADTLSTMF KMRMLRLEIG RRSFSSKTDV SALRNIGIIA HIDAGKTTTT
ERMLYFSGKT KRIGNVDEGD TITDFLPQER SRGITIQSAA ISFSWQNKFR INLIDTPGHA
DFTFEVIRSL KVLDGCVTIL DAVAGVEAQT EKVWKQAKGI PKICFINKMD RAGAGFSRTT
KELIAKLNTR VLLLNVPVFA NTSGPGEPVF EGVLDVVNKK VLRWTPQDPE RVSVEDIICN
SYYWNDLVKL RECLVETLSE FDEELVEYFL DTANGDYMIV PPELINKSVK KSLLANYAVP
VLCGASFRNI GVQPLLDAVV NYLPSPLEAK LPEFNTNVPV EVNKKEGSVI NNNKNLCVAQ
AFKVITDPIR GIMVYVRVYS GCLNSGFTVY NSTTGAKFKI GKLVIMHANV AEEVKQLKCG
EIGVLTGSTV SENVRTGDTI ITHSMKKDGL KSLNRVKELG LKINPIETPP PVFSVAVEPR
TLGNKKLMEE SIATIIREDP SLHVNVDEET GQTLLSGMGQ LHLEIAKDRL LNELKAEVEV
GKVMVSFKET LNACTKQSVL QTEDGYRFSI SVEPLDVAMA AKSSDEDWHY LSTDNNYLIL
EKSPACAGNW PYQISYEQIV NAVISSSIVA LQKSGKIANF PLHSCAVRIK NDWSIPLDAT
SVSQTLALSR GLIFEALREL PDTAFAVLEP IMHINVTVKQ SDMGHVLQDL TGARKANILS
IDDEHDLTSG ENADIQYQRL AEDQFLPMDA TLGLANISSG ATVNKTIRAL VPLKEIVSYM
SKLRSLTKGR GSYTMNYHGM EKVTSDRLPS ILEEF
//
