ID A0A1G4KIT0_9SACH Unreviewed; 4908 AA.
AC A0A1G4KIT0;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=Midasin {ECO:0000256|ARBA:ARBA00017143, ECO:0000256|PIRNR:PIRNR010340};
GN ORFNames=LAMI_0H16314G {ECO:0000313|EMBL:SCV04461.1};
OS Lachancea mirantina.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Lachancea.
OX NCBI_TaxID=1230905 {ECO:0000313|EMBL:SCV04461.1, ECO:0000313|Proteomes:UP000191024};
RN [1] {ECO:0000313|Proteomes:UP000191024}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Devillers H.;
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Nuclear chaperone required for maturation and nuclear export
CC of pre-60S ribosome subunits. {ECO:0000256|PIRNR:PIRNR010340}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000256|ARBA:ARBA00004604}. Nucleus, nucleoplasm
CC {ECO:0000256|ARBA:ARBA00004642}.
CC -!- SIMILARITY: Belongs to the midasin family.
CC {ECO:0000256|ARBA:ARBA00007188, ECO:0000256|PIRNR:PIRNR010340}.
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DR EMBL; LT598468; SCV04461.1; -; Genomic_DNA.
DR STRING; 1230905.A0A1G4KIT0; -.
DR OrthoDB; 1221059at2759; -.
DR Proteomes; UP000191024; Chromosome h.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR CDD; cd00009; AAA; 2.
DR CDD; cd01460; vWA_midasin; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 7.
DR Gene3D; 3.40.50.410; von Willebrand factor, type A domain; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR040848; AAA_lid_7.
DR InterPro; IPR011704; ATPase_dyneun-rel_AAA.
DR InterPro; IPR048617; MDN1_AAA_lid_4.
DR InterPro; IPR012099; Midasin.
DR InterPro; IPR041190; Midasin_AAA_lid_5.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR025662; Sigma_54_int_dom_ATP-bd_1.
DR InterPro; IPR002035; VWF_A.
DR InterPro; IPR036465; vWFA_dom_sf.
DR PANTHER; PTHR48103:SF2; MIDASIN; 1.
DR PANTHER; PTHR48103; MIDASIN-RELATED; 1.
DR Pfam; PF07728; AAA_5; 7.
DR Pfam; PF12775; AAA_7; 1.
DR Pfam; PF17865; AAA_lid_5; 1.
DR Pfam; PF17867; AAA_lid_7; 3.
DR Pfam; PF21108; MDN1_4th; 1.
DR PIRSF; PIRSF010340; Midasin; 1.
DR SMART; SM00382; AAA; 6.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 6.
DR SUPFAM; SSF53300; vWA-like; 1.
DR PROSITE; PS00675; SIGMA54_INTERACT_1; 1.
DR PROSITE; PS50234; VWFA; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PIRNR:PIRNR010340};
KW Chaperone {ECO:0000256|ARBA:ARBA00023186, ECO:0000256|PIRNR:PIRNR010340};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|PIRNR:PIRNR010340};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR010340};
KW Reference proteome {ECO:0000313|Proteomes:UP000191024}.
FT DOMAIN 4702..4897
FT /note="VWFA"
FT /evidence="ECO:0000259|PROSITE:PS50234"
FT REGION 4051..4597
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4091..4108
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4109..4154
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4155..4172
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4188..4205
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4206..4252
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4290..4304
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4316..4350
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4364..4382
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4386..4400
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4421..4451
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4452..4496
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4497..4514
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4518..4540
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 4545..4592
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4908 AA; 554304 MW; 546CC83745F25F79 CRC64;
MLENNNISLD LDVCRHRFES FNKFFEPHKL HTFHEIDLKA NVADSLCFLA EQILKSSSTT
AYLWIYQPIL LDIVARWASR RERQYYSSVI SAIGEIVALF PQTTGLVEFF LEHASGDLNE
LLQERKIGDD VHRTLLAFYR LLSWDRTRFV AYVRPEVFYQ VLRSAASSQI EKLLCCKILG
MHMNMTEKAQ IAMQKSFGCH AIAMRGVYGN DLGVDYQFLE LNEAKRIAKY SQLFSEPEGD
NHFKGTSRNV LLIYPSALSD LVVSVYGVLL PLTAESSRRN DIRDPLTLVP TTDALDVIRK
LALTMQRSKP VMLVGAAGSG KTFLVDQLSK AMGQTSQMVK IHLGEQTDVK LLLGTYTSGE
KPGTFEWKYG VLTTAVREGR WVLIEDIDRA PTEVLSILLT LLEKHEISIH SRGETIRAAN
GFQLISTIRT PEKQVSEDHH EILDSSLVGY RLWEKIHLPE MGDQNLELIL KTRFKSISSL
VPKLILAFNS VRSILTSPQF ISLNRGVQPR TITVRDLVKL CSRIEVLIAN SGIRSSSDLM
ESATYDSIFA EATDCFASAI GEFGALTPIV EAIGSTLEIP PSRTALFLTR HVPNFIDSVN
NVTIGRSILP KSSSNIRKKS VNVTSFAQTN HSLRLMEQVG VAVQMIEPVL LVGETGTGKT
TVVQEMAKSV NQKLTVLNLS QQTESGDLLG SFKPVNCRTL AIPIIENFEK LFPTTFSMKK
NERFYMMLHR CFNKSQWKNV VRLLKEANKM AEKLLSAEQG TEQQPSKVKK RKLDMHDKNM
LLKKWSEFDE TVKKFEKQYS SIENSFIFDF VEGSLVSAVR NGEWLLLDEI NLASQDALES
ISDLLSEPSS RSVLLTEKGQ AEPIKAHPNF RIFACMNPAT DVGKRDLPMG VRSRFTEIYV
HSPDRDITDL LSIIDRYVGK YAANDEWVGN DIAELYLRAK KLSESNQIVD GANQRPHFSI
RTLTRALIYV KDIILIYGLR RSLFEAFCMC FLTLLNEKSE ALLKPLIEEF ILGRLKNAKS
VISQIPPCPG PDFIQFKHYW MKRGSEERVE QPHYIITPFV ERNMLNLVRA TSSKRFPVLI
QGPTSAGKTS MIKYLAQITG NKVVRINNHE HTDLQEYLGT YIADETGSLK FAEGALVEAL
RKGYWIILDE LNLAPTDVLE ALNRLLDDNR ELLIPETQEV VHPHPNFMLF ATQNPPGLYG
GRKILSRAFR NRFLELHFDD IPQDELEIIL KERCQIAPSY AKKIVEVYRQ LSVQRSASRL
FEQGNSFATL RDLFRWALRD SVGYDELAAN GYMLLAERCR SLDEKKVVMS IIEKVMRVKL
DMSQYYHQLE NTELMGIETS VVWTEAMCRL SVLVEACLKN HEPVLLVGET GCGKTTICQL
LAQFRNQGLT TVNAHQNTET GDILGAQRPR RNRSELQVRL SALLVKCIGE SLEDIDLNEV
SLSKLIELYD GCDKSLVPLE IQSEVTSLRD GLQVLFEWND GPLVQAMKEG QLFLLDEISL
ADDSVLERLN SVLEPERTLF LSEKGGQGAL VRASTMFEFL ATMNPGGDYG KKELSPALRN
RFTEIWVPSM ENFNDVRKIV SSKLLKSLQF LVEPVVLFSE WFAKKYGHGK ADSGIVSLRD
ILVWVEFLNS STKTAKEPLA MLLEGAAMVF IDALGTNNTG FLSENSTALA NEKHQCVEAL
SNFASHDLAS FLRSDLHLEL TSEMLRIGAF DIERKTTNTE IPAFSLSAPT TAKNLMKVVR
AMQVKKPILL EGSPGVGKTS LITALAECTG NKLTRINLSE QTDLVDLFGS DVPGERAGEF
VWRDAPFLRA MQLGEWVLLD EMNLASQSVL EGLNACFDHR GQAYVPELDK SFLRHENFVV
FAAQNPQHQG GGRKGLPKSF VNRFNVVYVE MLTPEDLSLI ATHIYPEIET SLISKMIDVV
ANLEMEVSVR KQWGASGGPW EFNLRDVLRW LKLLNNKSFN NGVNPADFLD LILVQRFRTQ
ADKMKARALI ENTFGSCHKR DNYMKLGPDF MQANQEVAER NVLSKKSPRG GVRTKALQCN
IPIYESVLRC LKNNWPVILV GPSFSGKTEV IRFLADTFGA HVVEFSMNSD VDSMDLLGGF
EQVDLNRALA DAVENVKQVI TDYSIVNLIA PKSSAHDRGV VACLKLDSLL KSTSISTDNF
DEFYQRFNDF KSSTTSEHVN NLAREIENLH LALSNASSVR FKWYDGLLLQ AMERGDWMIL
DNANLCSPSV LDRLNSLLEA GGSMVINECN LEDGSPRVVK PHPDFRLFLT VNPKFGELSR
AMRNRGVELF LEDLNIRSTS FDKLAMGFPG NEERKRDVNE KGDKVLSERG SKPLSSLVSQ
DASLMYELAQ VHDILIISQM RACEKIAGVL SLSAIASVND WLENVSACKL YDEATAIEQI
SGFVQSANNS DLTPKLSQLL STSQAKAALT VDLKGLWSHQ AIMPLLNFYN LPLLQVEYPF
ISTAEPVYLF ETVCFLRYSF TSLQQTESRA LHSQPDKLTY LESSAARHLG RQLKGNFKFP
VFDILVKLRT FIEQRMSENR LFVDESFFCS LFKLCAIWIN VEKSAIRRND AKLRAHRDLI
QEWKNSSSLD NRSSDDFMGI IELFGRLTEF TRGRSMTLIW EEFRGTYPTS EKSWRKFEKL
LDITSKFDQV SVMLYSDSYE LIGSLRQFLL DTFKDILLER DMDFEAIMSE IERSIGNLAT
ISSQFLYERY HYFRQEFDVV ARLNVAHEKH FLSSTVLEVL PFASISAASI MNSQCKGNFY
PPIFDVLWTR DNGCGFISHT STLFDTTLFG SLVEKLTGLT HIPGCHLSQT KEDGSLLLRY
LIRNSEQITV NQMKSFKEEL LQIYSRVSKL HAGFPAEKEA SSKETSNSAY MLQFYKHVDK
LFLKPAIQSA KETESAQSVG KAWIIFSLGM LQLYVPDGAY DPAIFDYVSN ECFSIEKGSK
ESLIESWRFG RLILSGDSPI SIENVISKEI KTGIAVKPRI YRPHESIDML FDEWQAFISS
TADSIPVTNM LTSIESADVR ALSQANLFQQ NTSQFIERLN SSFKVYSDVN DLLSSFINGL
KFGTDLVLFG RDKSVERSPK SPLWALNPLV ISSTSKICEV FEEAEKFSKN QGLKGSVVEK
LMIFFLKLYK LHDKAETLKP VFQKALECLY LRWVVRREQA EKEALNQSGI YRYNHKDEDE
ESEIAKLFPN CNDEEGSSED TADEFESASY DLAQSYMQVF SSRSVSITEV AEVGAELTDS
LIKNGFSANN VSKNSNDLVS VVHHFVSKLD CLSENQNHIE ETLDFYRDHS LGEIRKAIKI
VESLWGSVKE FLKQWPDHAI LLDLFRVSQE FCHYSLDTPI ARSLQKIEQI YTYVTEWQKY
ASTKVSLQTH VVALTELIVS WRKLELKTWA ALFDTEDEGS RKRIGKWWYH LFETLMIARY
DTIEGNENSQ SLTTLLSSLN VFLSNCTYGE FETRVELLRA FIAHVEIDYG GESALYLALS
NFVTFYEQFL PIIRRAKTDA RKALEKEMKD IILLASWKDV NVDALKQSSK RSHNSLFKIV
RKYREVLNLE TKPLIESGIP PQDKPEACFN TLSEDRITGV DLDEAQAILQ QLPSWTSRPK
THQSMARTVR NMDRHIYSIV NENLPSFYTF ANDVCSEAKR LRDETPKVYD KKDKKKLASL
KTQKIRMIND TLKDLRSMGL KLAFRDDIRK VQLSVTSILA HTDCLRDTCI DGCDAYFFRL
LDLMPRLKVT LSSPTDDIPP VLLTKGLAAS ENLMYMLINI RPAMRKSAEI SSAIKNLHFE
LNLVAKSTNA LIDTSFKRAV RNAESISEWL PGLIDYAITT VEKCQSYVNC ESDLGFLFEA
KTWISGLQQD IGGLHYSDER LIKTVFDFMN YMTDLANKLT SCKSIYSFVF EMVASWIKQK
LSQDVETLEV KSESANNVGE LDRSLQNLTT SIMLTVQKLV EQFGRASEES DTWLTDTQRT
ISEALYVVQN SRVLSNLRAS TDIIIQSNLN KKSSKVVSYM CSYALPLLDS FRRLLTGILN
MASKNYENLS HGTFLLANML LILGSEGLCS PTPPSEEVDD KNLTDGTGLG EGDGAESKGD
GLDDEEINEV AQESNEKQKD KEDMDDKGED SAVEIEGDMG GDLEDASGED ESEGEDKDED
ENELDEEVDN IGDDDPNAID EKMWDNDGSS NSNEKESEQA LNNPANEEMK ASEEDHDKLN
EPHGDEGADE DQESNEAPGE GSEEDDREHD EGGDESEDDA VGQNDEVVDE QTADDMAPPP
PEVDTMNLPE GMNLDSEEEP ISDNEADIKS VDEMDVDEEN SEDHEAEANQ ISKEEEFNEE
ESDNLDEEES IEKTEELESS PEADEDNHDI EQFDQEVKNT KDDQTPTGES GGEDSLEGLN
GLNNSDVKME DEDHDTAVQQ EAGTRGEGGD ALDNEERNDI GSNGAADQSQ QMNQDSSAND
SSRQEANESL KQLGDNLKEF HRRHNEIREA SQSNTEENDQ ERANQRPDEF EHVEGANTED
TTQALGSANK DQVTSIDEEM AIDDEDLDQE EVEDAEAEFD TNNTGIEQDI EREPEKGPND
GDHRESEKKS SGAYIGERQH ISEKDKFTSS DLLGSSDDDL DQLMEEVDRK VKIDHDQLEP
ARSLEESRAL WRNSETQTAE LSAGLCEQLR LILEPTLATK LRGDYKTGKR LNMKKIIPYI
ASQFRKDKIW LRRTKPSKRQ YQIMIAVDDS KSMSESKSVN LAFQSICLVS KALTQLESGG
LSVVKFGETV QEVHGFNRAF SGDSGPKIFQ WFDFQESKTD VKKLVAESIR IFQQAQSSST
ADLWQLQIVI SDGVCEDHET ILRLVRRARE NRIMLVFVIV DGINSSQSIM DMSQVSYIPD
QSGNLQLKVG KYLDTFPFEF YVVVRDISEL PEMLSVILRQ YFSELAAA
//
