ID A0A1G4M8B4_LACFM Unreviewed; 775 AA.
AC A0A1G4M8B4;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 24-JAN-2024, entry version 31.
DE RecName: Full=General negative regulator of transcription subunit {ECO:0000256|PIRNR:PIRNR005290};
GN ORFNames=LAFE_0B05710G {ECO:0000313|EMBL:SCV99932.1};
OS Lachancea fermentati (Zygosaccharomyces fermentati).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Lachancea.
OX NCBI_TaxID=4955 {ECO:0000313|EMBL:SCV99932.1, ECO:0000313|Proteomes:UP000190831};
RN [1] {ECO:0000313|Proteomes:UP000190831}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Devillers H.;
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as component of the CCR4-NOT core complex, which in the
CC nucleus seems to be a general transcription factor, and in the
CC cytoplasm the major mRNA deadenylase involved in mRNA turnover. The NOT
CC protein subcomplex negatively regulates the basal and activated
CC transcription of many genes. Preferentially affects TC-type TATA
CC element-dependent transcription. Could directly or indirectly inhibit
CC component(s) of the general transcription machinery.
CC {ECO:0000256|PIRNR:PIRNR005290}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR005290}.
CC Nucleus {ECO:0000256|ARBA:ARBA00004123, ECO:0000256|PIRNR:PIRNR005290}.
CC -!- SIMILARITY: Belongs to the CNOT2/3/5 family.
CC {ECO:0000256|ARBA:ARBA00007682, ECO:0000256|PIRNR:PIRNR005290}.
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DR EMBL; LT598489; SCV99932.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G4M8B4; -.
DR STRING; 4955.A0A1G4M8B4; -.
DR OMA; VPPGIQD; -.
DR OrthoDB; 10289at2759; -.
DR Proteomes; UP000190831; Chromosome b.
DR GO; GO:0030015; C:CCR4-NOT core complex; IEA:UniProtKB-UniRule.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000932; C:P-body; IEA:UniProtKB-UniRule.
DR GO; GO:0000289; P:nuclear-transcribed mRNA poly(A) tail shortening; IEA:UniProtKB-UniRule.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR Gene3D; 2.30.30.1020; CCR4-NOT complex subunit 2/3/5, C-terminal domain; 1.
DR InterPro; IPR038635; CCR4-NOT_su2/3/5_C_sf.
DR InterPro; IPR012270; CCR4-NOT_su3/5.
DR InterPro; IPR040168; Not2/3/5.
DR InterPro; IPR007282; NOT2/3/5_C.
DR InterPro; IPR007207; Not_N.
DR PANTHER; PTHR23326; CCR4 NOT-RELATED; 1.
DR PANTHER; PTHR23326:SF16; GENERAL NEGATIVE REGULATOR OF TRANSCRIPTION SUBUNIT 3; 1.
DR Pfam; PF04153; NOT2_3_5_C; 1.
DR Pfam; PF04065; Not3; 1.
DR PIRSF; PIRSF005290; NOT_su_3_5; 2.
PE 3: Inferred from homology;
KW Activator {ECO:0000256|PIRNR:PIRNR005290};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|PIRNR:PIRNR005290};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|PIRNR:PIRNR005290};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000190831};
KW Repressor {ECO:0000256|ARBA:ARBA00022491, ECO:0000256|PIRNR:PIRNR005290};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|PIRNR:PIRNR005290};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW ECO:0000256|PIRNR:PIRNR005290}.
FT DOMAIN 3..233
FT /note="CCR4-Not complex component Not N-terminal"
FT /evidence="ECO:0000259|Pfam:PF04065"
FT DOMAIN 648..740
FT /note="NOT2/NOT3/NOT5 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF04153"
FT REGION 248..358
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 420..463
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 480..529
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 41..68
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 125..183
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 253..282
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 294..358
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 422..463
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 480..507
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 515..529
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 775 AA; 86802 MW; C1789E0AB25E7CDA CRC64;
MAHRKLQQEI DKVFKKINEG LEIFNTYYER HENCTNNPSQ KDKLEADLKR EVKKLQRLRE
QIKSWQSSPD IKDKDSLLDY RRSVEVAMEK YKAVEKASKE KAYSNMSLKK SETLDPEEME
RRDVTNYLSQ SIDELERQYD ALQVEADRLI LLNKKKKTAT VANEEKKNKL KEMQLRYRWH
QQQMELALRL IANEELNPQD VKDIEDDINF FIESNQEPDF VEDETIYDGL NLDSNEAIAH
EVAASFAAQM NDESEEESSK DSSKLSKKEQ RKLEREAKRA AKAAAKAVTP GEEHANISGQ
NDLTKSVDSV EQSKDSVSVS PSASPSPVPS SMGVSIPSTA NTTLPNGTST AGSSVGIVNR
NQGTQVKILS RESTPELQGH THIHQSLNGI TTTSTLKPAT VPVRPAGDLR WAVAATQAAE
REKKINASNG TSTISASSSN NNSRNTSVSN TPVLPTYSLS TSHEKPSLAS KVLAGAVTNG
QQQGNNQSSP IIKHSELSKQ TDVQDVPSSA MEPELVDDYD SEESDEDEPL NTEIIELSEE
EKQRREQVTN HLKHVLCDDS ELLLLPGGIQ DMIISSLLSR NKMEDANGRK SNIQTLSDAC
QVSRLEMIPS GVNPPSPLDA FRSTSQWDST RCSLRDEIAT IEGDDETKLK TLLPRFGPLE
TFSLFYNYYY SVTPLEKKIA YSLLTERDWK ISKSGDTWFL RHGNPKFSNE LCEVADFKIF
KLDDWTVVDK LNFKLDYSLL RNPPVTQPDV SNGQQLLQQL KQGGLLAKPG FVAAQ
//
