ID   A0A1G4MI13_LACFM        Unreviewed;       532 AA.
AC   A0A1G4MI13;
DT   18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT   18-JAN-2017, sequence version 1.
DT   27-MAR-2024, entry version 23.
DE   RecName: Full=tRNA (uracil-O(2)-)-methyltransferase {ECO:0000256|ARBA:ARBA00017788, ECO:0000256|RuleBase:RU368004};
DE            EC=2.1.1.211 {ECO:0000256|ARBA:ARBA00012795, ECO:0000256|RuleBase:RU368004};
GN   ORFNames=LAFE_0G12596G {ECO:0000313|EMBL:SCW03534.1};
OS   Lachancea fermentati (Zygosaccharomyces fermentati).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Lachancea.
OX   NCBI_TaxID=4955 {ECO:0000313|EMBL:SCW03534.1, ECO:0000313|Proteomes:UP000190831};
RN   [1] {ECO:0000313|EMBL:SCW03534.1, ECO:0000313|Proteomes:UP000190831}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CBS 6772 {ECO:0000313|EMBL:SCW03534.1};
RA   Devillers H.;
RL   Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-
CC       methyltransferase. {ECO:0000256|RuleBase:RU368004}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC         methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC         Evidence={ECO:0000256|ARBA:ARBA00000660,
CC         ECO:0000256|RuleBase:RU368004};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC       ECO:0000256|RuleBase:RU368004}.
CC   -!- SIMILARITY: Belongs to the TRM44 family.
CC       {ECO:0000256|ARBA:ARBA00009056, ECO:0000256|RuleBase:RU368004}.
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DR   EMBL; LT598486; SCW03534.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1G4MI13; -.
DR   STRING; 4955.A0A1G4MI13; -.
DR   OMA; IREPNIN; -.
DR   OrthoDB; 1353816at2759; -.
DR   Proteomes; UP000190831; Chromosome g.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0141101; F:tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR   InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR   PANTHER; PTHR21210:SF0; TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED; 1.
DR   PANTHER; PTHR21210; UNCHARACTERIZED; 1.
DR   Pfam; PF07757; AdoMet_MTase; 1.
PE   3: Inferred from homology;
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|RuleBase:RU368004};
KW   Methyltransferase {ECO:0000256|RuleBase:RU368004};
KW   Reference proteome {ECO:0000313|Proteomes:UP000190831};
KW   S-adenosyl-L-methionine {ECO:0000256|RuleBase:RU368004};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU368004};
KW   tRNA processing {ECO:0000256|RuleBase:RU368004}.
SQ   SEQUENCE   532 AA;  61203 MW;  FE6CBF6404B7F9FC CRC64;
     MKPRFEYDAQ GPSILGDGWY CMYKTQEPVS FKIDHFETAM LNVIRQPNIN STVILRADML
     KEWLYDVKTG ERLSYEAQNY EVTDADAQTI NLDDLEIRQV KVHPDLGLSI KRAMVRRIIP
     RNPYKDAIIN QTCLVMNSRQ FDETSLIVYT PHFERKEQCP FYIPQVRVVA ILLHKGQLSV
     HYIPFEIEDV AELHDETQRV VRTALRLLQT AHKHSTGVKA GYEKKVTHDL VVNKVAFQDR
     YIALKKKYSR FLVDNWAEST DPKKHVFEDI AIAAFLIELW IKVYGNNFKE KVEFRDLGCG
     NGVLCYLLIS EGIRGIGIDA RQRKSWKIYP AEVQNQLKEQ VIIPAILLRP HPQVKKRAPQ
     LEHNGRFFPI QVHDKIAPAT VLYSSADLLK SPHVNITEFP RDTFIIGNHS DELTCWIPLL
     GYPFMVIPCC SHNFSGQRMR YGVRKDQNKS SNSTYAGLVD RVEYVAARVG WEIEKEMLRI
     PSTRNAAIIG INNAHLPSWP TQEVYDTILD DGGADGWVEN TMALMKRSPR GH
//