ID   A0A1G4P8G5_9FIRM        Unreviewed;       472 AA.
AC   A0A1G4P8G5;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 22.
DE   SubName: Full=NOL1/NOP2/sun family putative RNA methylase {ECO:0000313|EMBL:SCW28484.1};
GN   ORFNames=SAMN02910456_00343 {ECO:0000313|EMBL:SCW28484.1};
OS   Ruminococcaceae bacterium YRB3002.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
OX   NCBI_TaxID=1520819 {ECO:0000313|EMBL:SCW28484.1, ECO:0000313|Proteomes:UP000199293};
RN   [1] {ECO:0000313|EMBL:SCW28484.1, ECO:0000313|Proteomes:UP000199293}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YRB3002 {ECO:0000313|EMBL:SCW28484.1,
RC   ECO:0000313|Proteomes:UP000199293};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. RsmB/NOP family. {ECO:0000256|ARBA:ARBA00007494,
CC       ECO:0000256|PROSITE-ProRule:PRU01023}.
CC   -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC       feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01023}.
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DR   EMBL; FMTG01000002; SCW28484.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1G4P8G5; -.
DR   STRING; 1520819.SAMN02910456_00343; -.
DR   OrthoDB; 9810297at2; -.
DR   Proteomes; UP000199293; Unassembled WGS sequence.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR   GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR   CDD; cd02440; AdoMet_MTases; 1.
DR   Gene3D; 2.30.130.60; -; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR018314; Fmu/NOL1/Nop2p_CS.
DR   InterPro; IPR031341; Methyltr_RsmF_N.
DR   InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR   InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR   InterPro; IPR027391; Nol1_Nop2_Fmu_2.
DR   InterPro; IPR023267; RCMT.
DR   InterPro; IPR031340; RsmF_methylt_CI.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PANTHER; PTHR22807:SF30; 28S RRNA (CYTOSINE(4447)-C(5))-METHYLTRANSFERASE-RELATED; 1.
DR   PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR   Pfam; PF01189; Methyltr_RsmB-F; 1.
DR   Pfam; PF17125; Methyltr_RsmF_N; 1.
DR   Pfam; PF13636; Methyltranf_PUA; 1.
DR   Pfam; PF17126; RsmF_methylt_CI; 1.
DR   PRINTS; PR02008; RCMTFAMILY.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS01153; NOL1_NOP2_SUN; 1.
DR   PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE   3: Inferred from homology;
KW   Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW   ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000199293};
KW   RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW   ProRule:PRU01023};
KW   S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW   ECO:0000256|PROSITE-ProRule:PRU01023};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW   ProRule:PRU01023}.
FT   DOMAIN          24..295
FT                   /note="SAM-dependent MTase RsmB/NOP-type"
FT                   /evidence="ECO:0000259|PROSITE:PS51686"
FT   ACT_SITE        235
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         113..119
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         137
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT   BINDING         182
FT                   /ligand="S-adenosyl-L-methionine"
FT                   /ligand_id="ChEBI:CHEBI:59789"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ   SEQUENCE   472 AA;  52550 MW;  1DCCF85A2D4D95EC CRC64;
     MNLPEEFLAR MRDELGDDAS FEAFVASYDR SPLKGIRLST AKLTPDEYEH TAEILTGSHN
     RVPWTSCGYY SENETSGKDP YYHAGIYYPQ EPSAMLPAEV LAARPGEIIL DLCAAPGGKA
     CRIGEDLKGK GLLVANEINA ERSKALNRNI ERTGITNCII LNESPENIAS KLPVFFDKIL
     IDAPCSGEGM FRRDPRAVTS WKEYGPASIC KVQREILDQA VLMLKSGGDI VYSTCTFARV
     EDEDMIKGFL EEHVDFEIVP HREIDGVTHN DDGSMRIWPH LAEGDGHFCV HLRHKGDKSG
     SAYDLTPVPS SGSVNIKIKI AMMRYMSELL TADAYDELMR LCDERFVVQG AGLYLVPVDL
     RLFNGLKMIK SGLFLGEVKT TKTERVFTMS NSLPLALNSN MIREDSLISL ERDSDMLIRY
     LKGETITIDM SGYPQLRRKG VVVIAVDGHP LGLGKIDNGT VKNMYPKAWR LV
//