ID   A0A1G4PH14_9FIRM        Unreviewed;       295 AA.
AC   A0A1G4PH14;
DT   22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2017, sequence version 1.
DT   27-MAR-2024, entry version 18.
DE   RecName: Full=Methylenetetrahydrofolate reductase {ECO:0000256|RuleBase:RU003862};
GN   ORFNames=SAMN02910456_00498 {ECO:0000313|EMBL:SCW31592.1};
OS   Ruminococcaceae bacterium YRB3002.
OC   Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
OX   NCBI_TaxID=1520819 {ECO:0000313|EMBL:SCW31592.1, ECO:0000313|Proteomes:UP000199293};
RN   [1] {ECO:0000313|EMBL:SCW31592.1, ECO:0000313|Proteomes:UP000199293}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YRB3002 {ECO:0000313|EMBL:SCW31592.1,
RC   ECO:0000313|Proteomes:UP000199293};
RA   de Groot N.N.;
RL   Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5-methyl-5,6,7,8-tetrahydrofolate + NAD(+) = (6R)-5,10-
CC         methylene-5,6,7,8-tetrahydrofolate + H(+) + NADH;
CC         Xref=Rhea:RHEA:19821, ChEBI:CHEBI:15378, ChEBI:CHEBI:15636,
CC         ChEBI:CHEBI:18608, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.5.1.54;
CC         Evidence={ECO:0000256|ARBA:ARBA00034420};
CC       PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:19823;
CC         Evidence={ECO:0000256|ARBA:ARBA00034420};
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC         Evidence={ECO:0000256|ARBA:ARBA00001974,
CC         ECO:0000256|RuleBase:RU003862};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000256|ARBA:ARBA00004777, ECO:0000256|RuleBase:RU003862}.
CC   -!- SIMILARITY: Belongs to the methylenetetrahydrofolate reductase family.
CC       {ECO:0000256|ARBA:ARBA00006743, ECO:0000256|RuleBase:RU003862}.
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DR   EMBL; FMTG01000003; SCW31592.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1G4PH14; -.
DR   STRING; 1520819.SAMN02910456_00498; -.
DR   OrthoDB; 9812555at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000199293; Unassembled WGS sequence.
DR   GO; GO:0106312; F:methylenetetrahydrofolate reductase NADH activity; IEA:RHEA.
DR   GO; GO:0006555; P:methionine metabolic process; IEA:InterPro.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00537; MTHFR; 1.
DR   Gene3D; 3.20.20.220; -; 1.
DR   InterPro; IPR029041; FAD-linked_oxidoreductase-like.
DR   InterPro; IPR003171; Mehydrof_redctse-like.
DR   PANTHER; PTHR45754; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR   PANTHER; PTHR45754:SF3; METHYLENETETRAHYDROFOLATE REDUCTASE; 1.
DR   Pfam; PF02219; MTHFR; 1.
DR   SUPFAM; SSF51730; FAD-linked oxidoreductase; 1.
PE   3: Inferred from homology;
KW   FAD {ECO:0000256|ARBA:ARBA00022827, ECO:0000256|RuleBase:RU003862};
KW   Flavoprotein {ECO:0000256|ARBA:ARBA00022630,
KW   ECO:0000256|RuleBase:RU003862};
KW   Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW   ECO:0000256|RuleBase:RU003862};
KW   Reference proteome {ECO:0000313|Proteomes:UP000199293}.
SQ   SEQUENCE   295 AA;  32896 MW;  3F2BD30A559A709C CRC64;
     MKITDILNRG EMSLSFEVFP PKKETSFENV MNAAGQIAEL NPSFMSVTYG AGGGTSDYTL
     KLSSEIEHKY GVPILAHLTC VSSDRETVRE RILELKNAGI SNIMALRGDI PADMMDSDRS
     GWDYHYAVEL IREIKSIYPE CCIGAACYPE IHPESRNQRD DLLHLKEKVD AGVDFLTTQM
     VFDNNLFFNF LYKIRDMGVT VPVLPGIMPI TKANQVERAI KLSGSYMPVR FRTIVDRFGP
     DDDAMTQAGI IYATDQIIDL YANGITNVHV YSMNKPEVAA GIKNSLSSIL GREFA
//