ID A0A1G4W099_9MICO Unreviewed; 849 AA.
AC A0A1G4W099;
DT 18-JAN-2017, integrated into UniProtKB/TrEMBL.
DT 18-JAN-2017, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE SubName: Full=Membrane carboxypeptidase (Penicillin-binding protein) {ECO:0000313|EMBL:SCX14734.1};
GN ORFNames=SAMN05216534_1564 {ECO:0000313|EMBL:SCX14734.1};
OS Candidatus Aquiluna sp. UB-MaderosW2red.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Luna cluster; Luna-1 subcluster; Aquiluna.
OX NCBI_TaxID=1855377 {ECO:0000313|EMBL:SCX14734.1, ECO:0000313|Proteomes:UP000243284};
RN [1] {ECO:0000313|Proteomes:UP000243284}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UB-MaderosW2red {ECO:0000313|Proteomes:UP000243284};
RA Varghese N., Submissions S.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-
CC Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-
CC (1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-
CC cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-
CC D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans-octa-cis-undecaprenyl
CC diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+);
CC Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602, Rhea:RHEA-COMP:9603,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58405, ChEBI:CHEBI:60033,
CC ChEBI:CHEBI:78435; EC=2.4.1.129;
CC Evidence={ECO:0000256|ARBA:ARBA00023988};
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DR EMBL; LT627734; SCX14734.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G4W099; -.
DR STRING; 1855377.SAMN05216534_1564; -.
DR OrthoDB; 9766909at2; -.
DR Proteomes; UP000243284; Chromosome i.
DR GO; GO:0004180; F:carboxypeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd06577; PASTA_pknB; 2.
DR Gene3D; 3.30.10.20; -; 2.
DR Gene3D; 1.10.3810.10; Biosynthetic peptidoglycan transglycosylase-like; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR001264; Glyco_trans_51.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR005543; PASTA_dom.
DR InterPro; IPR036950; PBP_transglycosylase.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR PANTHER; PTHR32282; BINDING PROTEIN TRANSPEPTIDASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR32282:SF33; PEPTIDOGLYCAN GLYCOSYLTRANSFERASE; 1.
DR Pfam; PF03793; PASTA; 2.
DR Pfam; PF00912; Transgly; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SMART; SM00740; PASTA; 2.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF53955; Lysozyme-like; 1.
DR PROSITE; PS51178; PASTA; 1.
PE 4: Predicted;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645,
KW ECO:0000313|EMBL:SCX14734.1}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000243284}.
FT DOMAIN 706..773
FT /note="PASTA"
FT /evidence="ECO:0000259|PROSITE:PS51178"
SQ SEQUENCE 849 AA; 90555 MW; 6A4A019C26D60A6F CRC64;
MSGKSKGSAL TTFLKFGLFS AVAGVLSVAL LAPAVAVAGV AASTGIGMFE GLPSYIRPVN
SAESSTVYAL KNGVPVKMAT FFHENRVEVP FEDISPNLVN AVIATEDPRF YEHGGVDIIS
LVRAALTNLA TFGDGPGAST ITMQYVRQSL VEAANLSGDA SAIAAATEVT IERKLREIRL
SIALEQQFSK KDILAGYLNL VFLGNQINGV ETASQYYFGI SAKDLNIPQA AYLAAMLKSP
NDYKPDEAAN LERGIGRRNY VLQQMADAGY ISFEQAESYK TSPIQTKITK TRQGCQENQV
TAFFCDFTVW TIRNSPEFGV TPEEREILLR RGGLEIYTSL DLSVQQVAWD TTMEDLPPEN
EWGFGTASVS VEVGTGRIIS MAQNRYFDQS TEPVLGGTSV NYNTDKNYGG SSGFQPGSTY
KVFTLAQWLK DGHTLGEHVD GRIFTGEDVD LDGKPDPVKI WNVEKDFRAS CGGVAGLWEV
NNSGDKTVDD ISVFQAVVTS QNTAFAAMAA QLDLCAIRDT AAAFGVHRAD GAELEYFPAS
ILGTNEISPL AMAAAYAGIS NAGLYCSPVA IDKVLLRRTK EELPVPKTQC SQAVTPEIAA
AMTQVMRSVV SGGTGAAANP GDGTPLAGKT GTTDRRIHTW MTGFSSKVAT ATWVGNVVGL
TSQSSKSING RAVSTIRHSI WKEIMREVDK HYEGEAFPSP DPKYVIAPMV TVPEVTGFEL
ESAKVQLVTA GLSMQVITRE VASSQPVGAI AYTTPEFGTP VPKGSIVKVF ISSGGKIVVP
NIAGMDYTNA FATLQELGLT PSFPQPSQLS LNSRCDPIWP NDLAIGTSPA VGTEVSNASA
ILVIPNKCG
//
