ID A0A1G5AJC2_9FIRM Unreviewed; 808 AA.
AC A0A1G5AJC2;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=SAMN02910370_00084 {ECO:0000313|EMBL:SCX77981.1};
OS Lachnospiraceae bacterium XPB1003.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae.
OX NCBI_TaxID=1520825 {ECO:0000313|EMBL:SCX77981.1, ECO:0000313|Proteomes:UP000199543};
RN [1] {ECO:0000313|EMBL:SCX77981.1, ECO:0000313|Proteomes:UP000199543}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=XPB1003 {ECO:0000313|EMBL:SCX77981.1,
RC ECO:0000313|Proteomes:UP000199543};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|PROSITE-ProRule:PRU10059, ECO:0000256|RuleBase:RU361166}.
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DR EMBL; FMVK01000001; SCX77981.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A1G5AJC2; -.
DR STRING; 1520825.SAMN02910370_00084; -.
DR Proteomes; UP000199543; Unassembled WGS sequence.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR CDD; cd02850; E_set_Cellulase_N; 1.
DR Gene3D; 1.50.10.10; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR004197; Cellulase_Ig-like.
DR InterPro; IPR003305; CenC_carb-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF02018; CBM_4_9; 1.
DR Pfam; PF02927; CelD_N; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Reference proteome {ECO:0000313|Proteomes:UP000199543};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 24..808
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5039747306"
FT DOMAIN 88..213
FT /note="CBM-cenC"
FT /evidence="ECO:0000259|Pfam:PF02018"
FT DOMAIN 255..330
FT /note="Cellulase Ig-like"
FT /evidence="ECO:0000259|Pfam:PF02927"
FT DOMAIN 344..803
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT REGION 28..88
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 599..618
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 29..56
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 603..617
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 738
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 781
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 790
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 808 AA; 87460 MW; 2A016DFCEFF1F801 CRC64;
MKKKKMRGIA LVTAIALTSA GCAADKNPAT PVNSASEGNA ATQTVSSENG TGFNGPMVGQ
TAPRGAATTD DNIPVPEEGS APAPKTGDTL INIDFEDGNT GDFMTFTNGG DLELTNTARQ
LDCSINKCGS VDYGNQAYLD GFELVQGCEY TYSFDICSDI ERTVEYRLQL NGGDYHAYQG
DYISISPEVL HFSVDFTMEE ASDPAPRLVF NMGKMKDMSA DPGDHHVFID NIRLTVKNAD
NASLAGAGSL PAYLNVNTNQ VGFRPDDTKT VFVKTENDGE DFFVCRTSDS SIIWQGKLSE
AADDPASRAK TARGDFSEVS EPGSYYIYTA SGVSYPFTIG EDVYNGLYKD VVSMLYRQRC
GMALEGASAG AYAHEACHTA PAVIYGTNTT KDVSGGWHDA GDYGRYVVPG AKTVADLFAA
YEDYSLTSDD MGIPESGNGF PDLLDEAKYE LDWMLKMQDE KTGGVYHKVT CSNFPAMDAA
PEEETEELII SPISVTATGD FAAVMAKASV IYKDRDPEFA KTAYGAAVKA WDYIADKEDT
IGFLNPDGVE TGEYPDAVTA DERYWATAEL YLACLASAPG SENDDPAKYS SRLEELARQY
DEEKATQNSQ AGSDNASPDN RLGWADIISY AWYDLAKSDT EFSSAAKTNL TNEADKMLEA
SQKDRYFMCL GTDYYWGSNM GIASDAELLY MAANISDDAK AAEYRSLASR ALDYLLGANA
LGYSFITGYG TFFPKDIHHR PSLAAGNVMP GLLAGGADNR MEDPYAESVL SEAAPSMCYV
DNSQSYSTNE IAIYWNSPFI YILSAEMK
//
